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Fig. 6 | Biotechnology for Biofuels

Fig. 6

From: Genetic engineering of the Calvin cycle toward enhanced photosynthetic CO2 fixation in microalgae

Fig. 6

Evaluation of impacts of chloroplastic aldolase overexpression on the Calvin cycle. a Relative transcript levels of key enzymes involved in the Calvin cycle. The expression levels detected by real-time quantitative PCR are expressed as a ratio to wild-type (WT) cells, which was set to 1. b Measurement of activities of enzymes involved in the Calvin cycle. Samples were prepared from the exponentially growing cultures of C. vulgaris under normal culture conditions. For Rubisco enzyme, slash bar indicates the initial activity. Error bars represent SD (n = 3). Asterisks show significant difference from WT cells (t test, *P < 0.05)

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