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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: The TcEG1 beetle (Tribolium castaneum) cellulase produced in transgenic switchgrass is active at alkaline pH and auto-hydrolyzes biomass for increased cellobiose release

Fig. 1

Transformation vector map and relative transcript abundance of TcEG1 in transgenic switchgrass. a pANIC-10A-TcEG1 vector used for expression of TcEG1 in transgenic switchgrass. LB: left border; PvUbi2: switchgrass ubiquitin 2 promoter and intron; hph: hygromycin B phosphotransferase coding region; 35S T: 35S terminator sequence; PvUbi1: switchgrass ubiquitin 1 promoter and intron; pporRFP: Porites porites orange fluorescent protein coding region; NOS T: Agrobacterium tumefaciens nos terminator sequence; ZmUbi1: maize ubiquitin 1 promoter; R1 and R2: attR1 and attR2 recombinase sites 1 and 2; TcEG1: TcEG1 cDNA open reading frame; AcV5: epitope tag; RB: right border; Kanr: kanamycin resistance gene; ColE1: origin of replication in E. coli; pVS1: origin of replication in A. tumefaciens; OCS T: octopine synthase terminator sequence. b Relative transcript abundance of TcEG1 in stem internodes from transgenic events (Tc-1 to Tc-12). Relative expression analysis was determined by qRT-PCR and normalized to switchgrass ubiquitin 1 (PvUbi1). Bars represent mean values of three replicates ± standard error. Bars represented by different letters are significantly different as calculated by LSD (p ≤ 0.05)

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