Fig. 1
The pNsAtWRI1 vector and identification of the plasmid in the transformants. a Schematic map of the pNsAtWRI1 plasmid. b Detection of plasmids in NsAtWRI1 and WT. Genomic sequences of AtWRI1 (1.3 kb) and 18S rDNA (380 bp) were PCR amplified and the products were electrophoresed on agarose gels. c Western blotting of FLAG-tagged NsAtWRI1. The expected size of FLAG-tagged AtWRI1 was 49.4 kD, but running around 55 kD. AtpB (the CF1 ß subunit of ATP synthase of expected size 72.6 kD) was used as a loading control. d Relative intensity of AtWRI1-FLAG protein, which was calculated as the ratio of AtWRI1-FLAG vs AtpB. WT wild type, N normal conditions, NL nitrogen limitation, O osmotic stress