Table 1 Lipid composition (percentage of dry weight biomass, %) in WT and NsAtWRI1 transformants under different culture conditions analyzed by HPLC
Culture condition | Day | Strain | Neutral lipid (%) | Glycolipid (%) | Phospholipid (%) | |||
|---|---|---|---|---|---|---|---|---|
Hydrocarbon | TAG | Totala | MGDG | DGDG | PI | |||
Normal | Day 8 | WT | 3.7 ± 2.3 | 1.0 ± 1.7 | 4.7 ± 3.2 | 19.3 ± 2.5 | 1.2 ± 1.4 | n.d. |
|  |  | 2–3 | 7.1 ± 1.0* | 4.8 ± 0.7* | 11.9 ± 1.7* | 21.5 ± 2.0 | n.d. | n.d. |
|  |  | 1–31 | 1.8 ± 3.1 | 5.6 ± 0.7** | 7.3 ± 2.4 | 20.4 ± 1.4 | 3.1 ± 0.3 | n.d. |
|  | Day 12 | WT | 2.8 ± 0.3 | 15.0 ± 2.3 | 17.8 ± 2.6 | 11.1 ± 1.5 | 3.1 ± 1.8 | 0.5 ± 0.0 |
|  |  | 2–3 | 3.3 ± 0.3* | 27.0 ± 2.9*** | 30.3 ± 2.8*** | 11.1 ± 1.2 | 3.3 ± 1.4 | 0.2 ± 0.2 |
|  |  | 1–31 | 3.1 ± 0.2 | 18.6 ± 1.4* | 21.6 ± 1.4* | 11.0 ± 0.5 | 5.2 ± 0.4 | 0.8 ± 0.1 |
N limitation | Day 8 | WT | 5.8 ± 3.7 | 52.5 ± 6.9 | 58.3 ± 7.7 | n.d. | n.d. | n.d. |
|  |  | 2–3 | 8.7 ± 1.3 | 63.4 ± 6.8* | 72.0 ± 7.9* | n.d. | n.d. | n.d. |
|  |  | 1–31 | 9.3 ± 0.7 | 58.3 ± 2.4 | 67.6 ± 2.8* | n.d. | n.d. | n.d. |
|  | Day 12 | WT | 6.6 ± 1.3 | 72.7 ± 3.4 | 79.3 ± 2.3 | n.d. | n.d. | n.d. |
|  |  | 2–3 | 7.1 ± 0.3 | 79.1 ± 3.1* | 86.2 ± 3.2* | n.d. | n.d. | n.d. |
|  |  | 1–31 | 5.7 ± 3.4 | 73.8 ± 6.3 | 79.5 ± 5.3 | n.d. | n.d. | n.d. |
High salt | Day 8 | WT | 10.9 ± 10.9 | 26.8 ± 7.6 | 37.7 ± 3.5 | n.d. | 2.6 ± 4.5 | n.d. |
|  |  | 2–3 | 9.3 ± 11.6 | 31.7 ± 17.9 | 41.0 ± 14.1 | n.d. | 6.7 ± 11.7 | n.d. |
|  |  | 1–31 | 20.5 ± 5.5 | 17.3 ± 4.3 | 37.8 ± 9.8 | n.d. | 4.9 ± 8.4 | n.d. |
|  | Day 12 | WT | 5.7 ± 0.9 | 34.5 ± 4.9 | 40.2 ± 4.7 | 3.9 ± 3.9 | 4.2 ± 1.4 | 0.2 ± 0.4 |
|  |  | 2–3 | 6.7 ± 0.6 | 56.9 ± 5.7** | 63.6 ± 6.2** | 4.4 ± 4.4 | 2.1 ± 2.8 | n.d. |
|  |  | 1–31 | 6.4 ± 0.9 | 45.9 ± 11.0 | 52.2 ± 11.7 | 4.5 ± 4.5 | 3.6 ± 1.5 | 0.2 ± 0.4 |