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Table 1 Lipid composition (percentage of dry weight biomass, %) in WT and NsAtWRI1 transformants under different culture conditions analyzed by HPLC

From: Increased lipid production by heterologous expression of AtWRI1 transcription factor in Nannochloropsis salina

Culture condition Day Strain Neutral lipid (%) Glycolipid (%) Phospholipid (%)
Hydrocarbon TAG Totala MGDG DGDG PI
Normal Day 8 WT 3.7 ± 2.3 1.0 ± 1.7 4.7 ± 3.2 19.3 ± 2.5 1.2 ± 1.4 n.d.
   2–3 7.1 ± 1.0* 4.8 ± 0.7* 11.9 ± 1.7* 21.5 ± 2.0 n.d. n.d.
   1–31 1.8 ± 3.1 5.6 ± 0.7** 7.3 ± 2.4 20.4 ± 1.4 3.1 ± 0.3 n.d.
  Day 12 WT 2.8 ± 0.3 15.0 ± 2.3 17.8 ± 2.6 11.1 ± 1.5 3.1 ± 1.8 0.5 ± 0.0
   2–3 3.3 ± 0.3* 27.0 ± 2.9*** 30.3 ± 2.8*** 11.1 ± 1.2 3.3 ± 1.4 0.2 ± 0.2
   1–31 3.1 ± 0.2 18.6 ± 1.4* 21.6 ± 1.4* 11.0 ± 0.5 5.2 ± 0.4 0.8 ± 0.1
N limitation Day 8 WT 5.8 ± 3.7 52.5 ± 6.9 58.3 ± 7.7 n.d. n.d. n.d.
   2–3 8.7 ± 1.3 63.4 ± 6.8* 72.0 ± 7.9* n.d. n.d. n.d.
   1–31 9.3 ± 0.7 58.3 ± 2.4 67.6 ± 2.8* n.d. n.d. n.d.
  Day 12 WT 6.6 ± 1.3 72.7 ± 3.4 79.3 ± 2.3 n.d. n.d. n.d.
   2–3 7.1 ± 0.3 79.1 ± 3.1* 86.2 ± 3.2* n.d. n.d. n.d.
   1–31 5.7 ± 3.4 73.8 ± 6.3 79.5 ± 5.3 n.d. n.d. n.d.
High salt Day 8 WT 10.9 ± 10.9 26.8 ± 7.6 37.7 ± 3.5 n.d. 2.6 ± 4.5 n.d.
   2–3 9.3 ± 11.6 31.7 ± 17.9 41.0 ± 14.1 n.d. 6.7 ± 11.7 n.d.
   1–31 20.5 ± 5.5 17.3 ± 4.3 37.8 ± 9.8 n.d. 4.9 ± 8.4 n.d.
  Day 12 WT 5.7 ± 0.9 34.5 ± 4.9 40.2 ± 4.7 3.9 ± 3.9 4.2 ± 1.4 0.2 ± 0.4
   2–3 6.7 ± 0.6 56.9 ± 5.7** 63.6 ± 6.2** 4.4 ± 4.4 2.1 ± 2.8 n.d.
   1–31 6.4 ± 0.9 45.9 ± 11.0 52.2 ± 11.7 4.5 ± 4.5 3.6 ± 1.5 0.2 ± 0.4
  1. Analyses of lipid composition were performed using cells on day 8 and day 12. The data points represent the average of samples and error bars indicate standard deviation (n = 4). Significant differences against WT for the same condition, as determined by Student’s t test, are indicated by asterisks (*P < 0.05, **P < 0.01, ***P < 0.001)
  2. TAG triacylglycerol, MGDG monogalactosyldiacylglycerol, DGDG digalactosyldiacylglycerol, PI phosphatidylinositol, n.d. not detected
  3. aTotal neutral lipid contents were calculated by sum of hydrocarbon and TAG contents