Fig. 2
Time-course of fed-batch fermentation by strains RH01 and RH02 from xylose. a RH01; b RH02. The fermentations were conducted in a 5-L bioreactor with 2.5 L of initial medium. The initial concentration of xylose was approximately 57.0 g/L. The cultivation was performed at 50 °C with stirring at 80 rpm. The pH was maintained at 7.0 by automatic addition of 25% (w/v) Ca(OH)2. Xylose was added to the medium when its concentration was lower than 20.0 g/L. Each fermentation condition was repeated at least twice, and typical fermentation kinetics are shown here