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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: RETRACTED ARTICLE: Engineering Bacillus licheniformis as a thermophilic platform for the production of l-lactic acid from lignocellulose-derived sugars

Fig. 4

Time course of fed-batch fermentation of l-lactic acid by B. licheniformis strain RH15. a Mixed-sugar (glucose: xylose = 3:1) was used as a substrate; the initial concentrations of glucose and xylose were approximately 60.0 and 20.0 g/L, respectively; b Corn stover hydrolysate was used as the substrate. The fermentations were conducted in a 5-L bioreactor with 2.5 L of initial medium. The cultivation was performed at 50 °C with stirring at 80 rpm. The pH was maintained at 7.0 by automatic addition of 25% (w/v) Ca(OH)2. Mixed sugar (the ratio of glucose to xylose was approximately 3:1) or corn stover hydrolysate (700 g/L of sugars) was added to the medium when the glucose concentration was lower than 20.0 g/L. Each fermentation condition was repeated at least twice, and typical fermentation kinetics are shown here

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