Strain
|
Escherichia coli DH5α
|
Commercial transformation host for cloning
|
Novagen
|
E. coli S17-1
|
conjugative strain able to host λ-pir-dependent plasmids
|
In lab
|
B. licheniformis MW3
|
B. licheniformis ATCC 14580 ΔhsdR1ΔhsdR2
|
[20]
|
RH01
|
MW3 ΔalsSΔalsD
|
This study
|
RH02
|
RH01 Δldh::ldh
Bc
|
This study
|
RH03
|
RH01 Δldh::ldh
Bc
ΔptsG
|
This study
|
RH04
|
RH01 Δldh::ldh
Bc
ΔptsG::galP
|
This study
|
RH05
|
RH01 Δldh::ldh
Bc
ΔptsG::galP P
glcK
::P
als
a
|
This study
|
RH06
|
RH01 Δldh::ldh
Bc
ΔptsG::galP P
glcK
::P
als
ΔxylR
|
This study
|
RH07
|
RH01 Δldh::ldh
Bc
ΔptsG::galP P
glcK
::P
als
ΔxylR ΔxylA
Bl
::xylA
Tc
|
This study
|
RH08
|
RH01 Δldh::ldh
Bc
ΔptsG::galP P
glcK
::P
als
ΔxylA
Bl
::xylA
Bc
|
This study
|
RH09
|
RH01 Δldh::ldh
Bc
ΔptsG::galP P
glcK
::P
als
ΔxylA
Bl
::xylA
Tx
|
This study
|
RH10
|
RH01 Δldh::ldh
Bc
ΔptsG::galP P
glcK
::P
als
ΔxylA
Bl
::xylA
Tc
|
This study
|
RH11
|
RH01 Δldh::ldh
Bc
ΔptsG::galP P
glcK
::P
als
ΔxylA
Bl
::xylA
Ml
|
This study
|
RH12
|
RH08 ΔpflA
|
This study
|
RH13
|
RH08 ΔadhB
|
This study
|
RH14
|
RH08 ΔackA
|
This study
|
RH15
|
RH08 ΔadhBΔackA
|
This study
|
Plasmid
|
pKVM1
|
Gene knockout vector, OriT, traJ, ery
r
, amp
r
|
[9]
|
pKVM01
|
For deletion of alsS and alsD genes of strain MW3
|
This study
|
pKVM02
|
For replacement of ldh gene of strain RH01 with ldh
Bc
|
This study
|
pKVM03
|
For deletion of ptsG gene of strain RH02
|
This study
|
pKVM04
|
For replacement of ptsG gene of strain RH02 with galP
|
This study
|
pKVM05
|
For replacement of P
glcK
of strain RH04 with P
als
|
This study
|
pKVM06
|
For deletion of xylR gene of strain RH05
|
This study
|
pKVM07
|
For replacement of xylA
Bl
gene of strain RH06 with xylA
Tc
|
This study
|
pKVM08
|
For replacement of xylA
Bl
gene of strain RH05 with xylA
Bc
|
This study
|
pKVM09
|
For replacement of xylA
Bl
gene of strain RH05 with xylA
Tx
|
This study
|
pKVM10
|
For replacement of xylA
Bl
gene of strain RH05 with xylA
Tc
|
This study
|
pKVM11
|
For replacement of xylA
Bl
gene of strain RH05 with xylA
Ml
|
This study
|
pKVM12
|
For deletion of pflA gene of strain RH08
|
This study
|
pKVM13
|
For deletion of adhB gene of strain RH08
|
This study
|
pKVM14
|
For deletion of ackA gene of strain RH08
|
This study
|