Strain and plasmid | Relevant characteristic | Source |
---|---|---|
Strain | ||
Escherichia coli DH5α | Commercial transformation host for cloning | Novagen |
E. coli S17-1 | conjugative strain able to host λ-pir-dependent plasmids | In lab |
B. licheniformis MW3 | B. licheniformis ATCC 14580 ΔhsdR1ΔhsdR2 | [20] |
RH01 | MW3 ΔalsSΔalsD | This study |
RH02 | RH01 Δldh::ldh Bc | This study |
RH03 | RH01 Δldh::ldh Bc ΔptsG | This study |
RH04 | RH01 Δldh::ldh Bc ΔptsG::galP | This study |
RH05 | RH01 Δldh::ldh Bc ΔptsG::galP P glcK ::P als a | This study |
RH06 | RH01 Δldh::ldh Bc ΔptsG::galP P glcK ::P als ΔxylR | This study |
RH07 | RH01 Δldh::ldh Bc ΔptsG::galP P glcK ::P als ΔxylR ΔxylA Bl ::xylA Tc | This study |
RH08 | RH01 Δldh::ldh Bc ΔptsG::galP P glcK ::P als ΔxylA Bl ::xylA Bc | This study |
RH09 | RH01 Δldh::ldh Bc ΔptsG::galP P glcK ::P als ΔxylA Bl ::xylA Tx | This study |
RH10 | RH01 Δldh::ldh Bc ΔptsG::galP P glcK ::P als ΔxylA Bl ::xylA Tc | This study |
RH11 | RH01 Δldh::ldh Bc ΔptsG::galP P glcK ::P als ΔxylA Bl ::xylA Ml | This study |
RH12 | RH08 ΔpflA | This study |
RH13 | RH08 ΔadhB | This study |
RH14 | RH08 ΔackA | This study |
RH15 | RH08 ΔadhBΔackA | This study |
Plasmid | ||
pKVM1 | Gene knockout vector, OriT, traJ, ery r , amp r | [9] |
pKVM01 | For deletion of alsS and alsD genes of strain MW3 | This study |
pKVM02 | For replacement of ldh gene of strain RH01 with ldh Bc | This study |
pKVM03 | For deletion of ptsG gene of strain RH02 | This study |
pKVM04 | For replacement of ptsG gene of strain RH02 with galP | This study |
pKVM05 | For replacement of P glcK of strain RH04 with P als | This study |
pKVM06 | For deletion of xylR gene of strain RH05 | This study |
pKVM07 | For replacement of xylA Bl gene of strain RH06 with xylA Tc | This study |
pKVM08 | For replacement of xylA Bl gene of strain RH05 with xylA Bc | This study |
pKVM09 | For replacement of xylA Bl gene of strain RH05 with xylA Tx | This study |
pKVM10 | For replacement of xylA Bl gene of strain RH05 with xylA Tc | This study |
pKVM11 | For replacement of xylA Bl gene of strain RH05 with xylA Ml | This study |
pKVM12 | For deletion of pflA gene of strain RH08 | This study |
pKVM13 | For deletion of adhB gene of strain RH08 | This study |
pKVM14 | For deletion of ackA gene of strain RH08 | This study |