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Table 1 Bacterial strains and plasmids used and constructed in this study

From: RETRACTED ARTICLE: Engineering Bacillus licheniformis as a thermophilic platform for the production of l-lactic acid from lignocellulose-derived sugars

Strain and plasmid

Relevant characteristic

Source

Strain

 Escherichia coli DH5α

Commercial transformation host for cloning

Novagen

 E. coli S17-1

conjugative strain able to host λ-pir-dependent plasmids

In lab

 B. licheniformis MW3

B. licheniformis ATCC 14580 ΔhsdR1ΔhsdR2

[20]

 RH01

MW3 ΔalsSΔalsD

This study

 RH02

RH01 Δldh::ldh Bc

This study

 RH03

RH01 Δldh::ldh Bc ΔptsG

This study

 RH04

RH01 Δldh::ldh Bc ΔptsG::galP

This study

 RH05

RH01 Δldh::ldh Bc ΔptsG::galP P glcK ::P als a

This study

 RH06

RH01 Δldh::ldh Bc ΔptsG::galP P glcK ::P als ΔxylR

This study

 RH07

RH01 Δldh::ldh Bc ΔptsG::galP P glcK ::P als ΔxylR ΔxylA Bl ::xylA Tc

This study

 RH08

RH01 Δldh::ldh Bc ΔptsG::galP P glcK ::P als ΔxylA Bl ::xylA Bc

This study

 RH09

RH01 Δldh::ldh Bc ΔptsG::galP P glcK ::P als ΔxylA Bl ::xylA Tx

This study

 RH10

RH01 Δldh::ldh Bc ΔptsG::galP P glcK ::P als ΔxylA Bl ::xylA Tc

This study

 RH11

RH01 Δldh::ldh Bc ΔptsG::galP P glcK ::P als ΔxylA Bl ::xylA Ml

This study

 RH12

RH08 ΔpflA

This study

 RH13

RH08 ΔadhB

This study

 RH14

RH08 ΔackA

This study

 RH15

RH08 ΔadhBΔackA

This study

Plasmid

 pKVM1

Gene knockout vector, OriT, traJ, ery r , amp r

[9]

 pKVM01

For deletion of alsS and alsD genes of strain MW3

This study

 pKVM02

For replacement of ldh gene of strain RH01 with ldh Bc

This study

 pKVM03

For deletion of ptsG gene of strain RH02

This study

 pKVM04

For replacement of ptsG gene of strain RH02 with galP

This study

 pKVM05

For replacement of P glcK of strain RH04 with P als

This study

 pKVM06

For deletion of xylR gene of strain RH05

This study

 pKVM07

For replacement of xylA Bl gene of strain RH06 with xylA Tc

This study

 pKVM08

For replacement of xylA Bl gene of strain RH05 with xylA Bc

This study

 pKVM09

For replacement of xylA Bl gene of strain RH05 with xylA Tx

This study

 pKVM10

For replacement of xylA Bl gene of strain RH05 with xylA Tc

This study

 pKVM11

For replacement of xylA Bl gene of strain RH05 with xylA Ml

This study

 pKVM12

For deletion of pflA gene of strain RH08

This study

 pKVM13

For deletion of adhB gene of strain RH08

This study

 pKVM14

For deletion of ackA gene of strain RH08

This study

  1. a P glcK promoter of glucokinase gene glcK