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Table 1 Bacterial strains and plasmids used and constructed in this study

From: RETRACTED ARTICLE: Engineering Bacillus licheniformis as a thermophilic platform for the production of l-lactic acid from lignocellulose-derived sugars

Strain and plasmid Relevant characteristic Source
Strain
 Escherichia coli DH5α Commercial transformation host for cloning Novagen
 E. coli S17-1 conjugative strain able to host λ-pir-dependent plasmids In lab
 B. licheniformis MW3 B. licheniformis ATCC 14580 ΔhsdR1ΔhsdR2 [20]
 RH01 MW3 ΔalsSΔalsD This study
 RH02 RH01 Δldh::ldh Bc This study
 RH03 RH01 Δldh::ldh Bc ΔptsG This study
 RH04 RH01 Δldh::ldh Bc ΔptsG::galP This study
 RH05 RH01 Δldh::ldh Bc ΔptsG::galP P glcK ::P als a This study
 RH06 RH01 Δldh::ldh Bc ΔptsG::galP P glcK ::P als ΔxylR This study
 RH07 RH01 Δldh::ldh Bc ΔptsG::galP P glcK ::P als ΔxylR ΔxylA Bl ::xylA Tc This study
 RH08 RH01 Δldh::ldh Bc ΔptsG::galP P glcK ::P als ΔxylA Bl ::xylA Bc This study
 RH09 RH01 Δldh::ldh Bc ΔptsG::galP P glcK ::P als ΔxylA Bl ::xylA Tx This study
 RH10 RH01 Δldh::ldh Bc ΔptsG::galP P glcK ::P als ΔxylA Bl ::xylA Tc This study
 RH11 RH01 Δldh::ldh Bc ΔptsG::galP P glcK ::P als ΔxylA Bl ::xylA Ml This study
 RH12 RH08 ΔpflA This study
 RH13 RH08 ΔadhB This study
 RH14 RH08 ΔackA This study
 RH15 RH08 ΔadhBΔackA This study
Plasmid
 pKVM1 Gene knockout vector, OriT, traJ, ery r , amp r [9]
 pKVM01 For deletion of alsS and alsD genes of strain MW3 This study
 pKVM02 For replacement of ldh gene of strain RH01 with ldh Bc This study
 pKVM03 For deletion of ptsG gene of strain RH02 This study
 pKVM04 For replacement of ptsG gene of strain RH02 with galP This study
 pKVM05 For replacement of P glcK of strain RH04 with P als This study
 pKVM06 For deletion of xylR gene of strain RH05 This study
 pKVM07 For replacement of xylA Bl gene of strain RH06 with xylA Tc This study
 pKVM08 For replacement of xylA Bl gene of strain RH05 with xylA Bc This study
 pKVM09 For replacement of xylA Bl gene of strain RH05 with xylA Tx This study
 pKVM10 For replacement of xylA Bl gene of strain RH05 with xylA Tc This study
 pKVM11 For replacement of xylA Bl gene of strain RH05 with xylA Ml This study
 pKVM12 For deletion of pflA gene of strain RH08 This study
 pKVM13 For deletion of adhB gene of strain RH08 This study
 pKVM14 For deletion of ackA gene of strain RH08 This study
  1. a P glcK promoter of glucokinase gene glcK
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Biotechnology for Biofuels

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