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Table 3 Effect of different genes knocked out on l-lactic acid production

From: RETRACTED ARTICLE: Engineering Bacillus licheniformis as a thermophilic platform for the production of l-lactic acid from lignocellulose-derived sugars

Strain Cell density (OD600) Sugar consumed (g/L) Products (g/L) Yield (%)
Glucose Xylose Glucose + xylose l-Lactic acid Formic acid Acetic acid Succinic acid Ethanol  
RH08 7.8 57.1 18.0 75.0 66.7 2.7 2.7 < 0.1 2.1 88.9
RH12 4.9 42.8 13.5 56.3 50.3 < 0.1 2.0 < 0.1 1.6 89.3
RH13 8.0 57.8 18.1 75.9 68.5 2.8 3.0 < 0.1 < 0.1 90.3
RH14 7.6 56.2 17.6 73.8 68.2 2.9 0.51 < 0.1 2.4 92.4
RH15 7.7 56.3 17.8 74.1 70.5 3.1 0.12 < 0.1 < 0.1 95.1
  1. The experiments were conducted in 5-L bioreactor with 2.5 L initial medium at 50 °C for 29 h. The initial concentration of mixture sugars was 80 g/L (glucose: xylose = 3: 1, w/w). The cultivation was carried out at 50 °C, stirring at 80 rpm. The pH was maintained at 7.0 by automatic addition of 25% (w/v) Ca(OH)2 using a program-controlled peristaltic pump