From: A new method to evaluate temperature vs. pH activity profiles for biotechnological relevant enzymes
Enzyme | Assay | Substrate | Substrate conc. [mg/ml] | Enzyme conc. [µg/ml] | Assay duration [min] | Temp. gradient [°C] | pH gradient |
---|---|---|---|---|---|---|---|
Cel8A | DNSA | BBG | 3.3 | 0.5 | 90 | 60.1 ± 19.9 | 4.0–8.0 |
Azo-CMC | Azo-CMC | 3.3 | 5 | 90 | 60.1 ± 19.9 | 4.0–8.0 | |
Celluclast® | DNSA | BBG | 3.3 | 5 | 90 | 55.0 ± 19.9 | 4.0–8.0 |
AX | 3.3 | 200 | 90 | 55.0 ± 19.9 | 4.0–8.0 | ||
p-NP | p-NP-β-d-gluco-pyranoside | 1.9 mM | 50 | 15 | 55.0 ± 19.9 | 4.0–8.0 | |
Glucose HK | Wheat straw | 10 | 250 | 90 | 55.0 ± 19.9 | 4.0–8.0 |