Skip to main content

Table 1 Overview of the different enzymes, substrates, and assay conditions

From: A new method to evaluate temperature vs. pH activity profiles for biotechnological relevant enzymes

Enzyme

Assay

Substrate

Substrate conc. [mg/ml]

Enzyme conc. [µg/ml]

Assay duration [min]

Temp. gradient [°C]

pH gradient

Cel8A

DNSA

BBG

3.3

0.5

90

60.1 ± 19.9

4.0–8.0

Azo-CMC

Azo-CMC

3.3

5

90

60.1 ± 19.9

4.0–8.0

Celluclast®

DNSA

BBG

3.3

5

90

55.0 ± 19.9

4.0–8.0

AX

3.3

200

90

55.0 ± 19.9

4.0–8.0

p-NP

p-NP-β-d-gluco-pyranoside

1.9 mM

50

15

55.0 ± 19.9

4.0–8.0

Glucose HK

Wheat straw

10

250

90

55.0 ± 19.9

4.0–8.0