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Table 1 Overview of the different enzymes, substrates, and assay conditions

From: A new method to evaluate temperature vs. pH activity profiles for biotechnological relevant enzymes

Enzyme Assay Substrate Substrate conc. [mg/ml] Enzyme conc. [µg/ml] Assay duration [min] Temp. gradient [°C] pH gradient
Cel8A DNSA BBG 3.3 0.5 90 60.1 ± 19.9 4.0–8.0
Azo-CMC Azo-CMC 3.3 5 90 60.1 ± 19.9 4.0–8.0
Celluclast® DNSA BBG 3.3 5 90 55.0 ± 19.9 4.0–8.0
AX 3.3 200 90 55.0 ± 19.9 4.0–8.0
p-NP p-NP-β-d-gluco-pyranoside 1.9 mM 50 15 55.0 ± 19.9 4.0–8.0
Glucose HK Wheat straw 10 250 90 55.0 ± 19.9 4.0–8.0