Fig. 1

Comparison of all β-1,4-glucanases from the C. thermocellum cellulosome (listed in first column). Second column: Intermediate and final product analysis of different cellulases on various cello-oligosaccharides and PASC as substrate. The arrays show the oligosaccharide products (degree of polymerization ranging from glucose DP 1 to cellohexaose DP 6) on the Y-axis and the kinetic product shift over time (X-axis). The sugar amount detected by thin-layer chromatography is depicted as heat map representation, with relative intensities of the sugar products ranging from 1% (light gray) to 100% (black). Explanations of time points: 0.5 = 0.5 min; 2 = 2 min; 5 = 5 min; ¼ h = 15 min; 1 h = 60 min; 2 h = 120 min; on = overnight incubation. Empty fields (white) indicate that no products were formed, or products were below the detection limit of thin-layer chromatography. The pattern of protein CtCel124 is not shown due to its low activity. Third column: Activity of recombinant cellulases on various substrates (average values from triplicate measurements) at optimal cellulosome activity parameters (60 °C, pH 5.8; see Additional file 6). Fourth column: presence of glycoside hydrolase (GH) families, carbohydrate-binding modules (CBM), and Ig-like modules (Ig). For continuation of this figure, please see Fig. 2