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Table 2 Design and experimental results of the L25(55) orthogonal array of Taguchi experimental design for β-glucosidase activity from B. subtilis RA10

From: Bioprospecting of novel thermostable β-glucosidase from Bacillus subtilis RA10 and its application in biomass hydrolysis

S. no. Carbon source Organic N source pH Surfactant Inorganic N source β-Glucosidase activity (IU/g)
1 Paddy straw Yeast extract 6 Tween 20 NH4Cl 25.00
2 Paddy straw Malt extract 7 Tween 80 NaNO3 32.37
3 Paddy straw Beef extract 8 Triton X 100 (NH4)2HPO4 31.85
4 Paddy straw Peptone 9 PEG 6000 NH4NO3 32.77
5 Paddy straw Tryptone 10 (NH4)2SO4 21.32
6 Wheat bran Yeast extract 8 Tween 80 NH4NO3 25.74
7 Wheat bran Malt extract 9 Triton X 100 (NH4)2SO4 22.26
8 Wheat bran Beef extract 10 PEG 6000 NH4Cl 19.37
9 Wheat bran Peptone 6 NaNO3 17.4
10 Wheat bran Tryptone 7 Tween 20 (NH4)2HPO4 21.32
11 Baggase Yeast extract 10 Triton X 100 NaNO3 26.47
12 Baggase Malt extract 6 PEG 6000 (NH4)2HPO4 17.12
13 Baggase Beef extract 7 NH4NO3 15.95
14 Baggase Peptone 8 Tween 20 (NH4)2SO4 20.06
15 Baggase Tryptone 9 Tween 80 NH4Cl 15.22
16 Wheat straw Yeast extract 7 PEG 6000 (NH4)2SO4 22.38
17 Wheat straw Malt extract 8 NH4Cl 16.76
18 Wheat straw Beef extract 9 Tween 20 NaNO3 33.95
19 Wheat straw Peptone 10 Tween 80 (NH4)2HPO4 27.02
20 Wheat straw Tryptone 6 Triton X 100 NH4NO3 21.82
21 Parthenium Yeast extract 9 (NH4)2HPO4 11.03
22 Parthenium Malt extract 10 Tween 20 NH4NO3 17.32
23 Parthenium Beef extract 6 Tween 80 (NH4)2SO4 16.32
24 Parthenium Peptone 7 Triton X 100 NH4Cl 19.63
25 Parthenium Tryptone 8 PEG 6000 NaNO3 15.82