Bioprospecting of novel thermostable β-glucosidase from Bacillus subtilis RA10 and its application in biomass hydrolysis

Table 2 Design and experimental results of the L₂₅(5⁵) orthogonal array of Taguchi experimental design for β-glucosidase activity from B. subtilis RA10

S. no.	Carbon source	Organic N source	pH	Surfactant	Inorganic N source	β-Glucosidase activity (IU/g)
1	Paddy straw	Yeast extract	6	Tween 20	NH₄Cl	25.00
2	Paddy straw	Malt extract	7	Tween 80	NaNO₃	32.37
3	Paddy straw	Beef extract	8	Triton X 100	(NH₄)₂HPO₄	31.85
4	Paddy straw	Peptone	9	PEG 6000	NH₄NO₃	32.77
5	Paddy straw	Tryptone	10	–	(NH₄)₂SO₄	21.32
6	Wheat bran	Yeast extract	8	Tween 80	NH₄NO₃	25.74
7	Wheat bran	Malt extract	9	Triton X 100	(NH₄)₂SO₄	22.26
8	Wheat bran	Beef extract	10	PEG 6000	NH₄Cl	19.37
9	Wheat bran	Peptone	6	–	NaNO₃	17.4
10	Wheat bran	Tryptone	7	Tween 20	(NH₄)₂HPO₄	21.32
11	Baggase	Yeast extract	10	Triton X 100	NaNO₃	26.47
12	Baggase	Malt extract	6	PEG 6000	(NH₄)₂HPO₄	17.12
13	Baggase	Beef extract	7	–	NH₄NO₃	15.95
14	Baggase	Peptone	8	Tween 20	(NH₄)₂SO₄	20.06
15	Baggase	Tryptone	9	Tween 80	NH₄Cl	15.22
16	Wheat straw	Yeast extract	7	PEG 6000	(NH₄)₂SO₄	22.38
17	Wheat straw	Malt extract	8	–	NH₄Cl	16.76
18	Wheat straw	Beef extract	9	Tween 20	NaNO₃	33.95
19	Wheat straw	Peptone	10	Tween 80	(NH₄)₂HPO₄	27.02
20	Wheat straw	Tryptone	6	Triton X 100	NH₄NO₃	21.82
21	Parthenium	Yeast extract	9	–	(NH₄)₂HPO₄	11.03
22	Parthenium	Malt extract	10	Tween 20	NH₄NO₃	17.32
23	Parthenium	Beef extract	6	Tween 80	(NH₄)₂SO₄	16.32
24	Parthenium	Peptone	7	Triton X 100	NH₄Cl	19.63
25	Parthenium	Tryptone	8	PEG 6000	NaNO₃	15.82

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