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Table 4 Box–Behnken design matrix for the experimental design and respective response for β-glucosidase activity from B. subtilis RA10

From: Bioprospecting of novel thermostable β-glucosidase from Bacillus subtilis RA10 and its application in biomass hydrolysis

Std. order Experimental coded values of different variables β-Glucosidase activity (IU/g)
Paddy straw Beef extract PEG 6000 NaNO3 Actual
1 1 0.1 0.25 0.25 37.67
2 3 0.1 0.25 0.25 47.12
3 1 0.4 0.25 0.25 30.41
4 3 0.4 0.25 0.25 33.15
5 2 0.25 0.1 0.1 36.48
6 2 0.25 0.4 0.1 34.42
7 2 0.25 0.1 0.4 42.97
8 2 0.25 0.4 0.4 44.52
9 1 0.25 0.25 0.1 36.85
10 3 0.25 0.25 0.1 42.95
11 1 0.25 0.25 0.4 46.64
12 3 0.25 0.25 0.4 54.74
13 2 0.1 0.1 0.25 37.33
14 2 0.4 0.1 0.25 26.71
15 2 0.1 0.4 0.25 35.57
16 2 0.4 0.4 0.25 24.95
17 1 0.25 0.1 0.25 38.72
18 3 0.25 0.1 0.25 34.15
19 1 0.25 0.4 0.25 27.29
20 3 0.25 0.4 0.25 43.05
21 2 0.1 0.25 0.1 41.42
22 2 0.4 0.25 0.1 28.55
23 2 0.1 0.25 0.4 48.96
24 2 0.4 0.25 0.4 40.60
25 2 0.25 0.25 0.25 37.03
26 2 0.25 0.25 0.25 37.03
27 2 0.25 0.25 0.25 37.03