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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: A seven-gene cluster in Ruminiclostridium cellulolyticum is essential for signalization, uptake and catabolism of the degradation products of cellulose hydrolysis

Fig. 4

Characterization of R. cellulolyticum wild-type and MTLcuaD strains. a Growth of the wild-type (black) and the mutant (red) strains on minimal medium containing 2 g L−1 arabinose (dotted line) or cellobiose (solid line). Experiments were performed in triplicate and bars indicate the standard deviation. b, c Western blot analyses of the membrane fraction obtained for wild-type, MTLcuaD strains, and cellulose adapted MTLcuaD strains, using anti-CuaA (b) or anti-CuaD (c) antiserum. The amounts of membrane fraction loaded are given in µg and are obtained from arabinose (ara) or cellobiose (cb) grown cells

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