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Fig. 6 | Biotechnology for Biofuels

Fig. 6

From: A seven-gene cluster in Ruminiclostridium cellulolyticum is essential for signalization, uptake and catabolism of the degradation products of cellulose hydrolysis

Fig. 6

Characterization of the adapted mutant strain. a Growth of R. cellulolyticum wild-type (black), MTLcuaD (red), and adapted MTLcuaD mutant strain (blue) on minimal medium containing cellobiose (2 g L−1). b Molecular analysis of the type II intron insertion in cuaD. PCR was performed using the primers pair CuaD_734Dir/CuaD_1187Rev on cells from wild-type (1), MTLcuaD mutant (2) or adapted MTLcuaD mutant (3) strains. Migration at 2.5 kb indicates intron insertion. c A deletion of two bases (in red) is observed in the stem part of the terminator located downstream cuaD (top panel and below left panel), thus reducing the strength of the terminator in adapted MTLcuaD strain (below right panel). The strength of the terminator is calculated using ARNold on line software

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