Fig. 7

Growth and analysis of R. cellulolyticum wild-type and MTLcuaD complemented strains. a Western blot using anti-CuaA antiserum. The amounts of membrane fractions loaded in the gel are given in µg. Membrane fractions were isolated from an arabinose (ara) or a cellobiose (cb) grown culture. b, c Growth curve of different strains grown in minimal medium containing 2 g L⁻¹ cellobiose (b) or 5 g L⁻¹ cellulose (c). The strains are WT strain (black), MTLcuaD strain (red); MTLcuaD strain carrying an empty vector (red-dotted line), MTLcuaD strain carrying pSOS-cbpA (blue), MTLcuaD strain carrying pSOSABC (purple), MTLcuaD strain carrying pSOSABC-cbpA (green). Experiments were performed in triplicate and bars indicate the standard deviation