Fig. 2
From: Modular pathway engineering for the microbial production of branched-chain fatty alcohols
Testing three alcohol formation modules. a Overall titer distribution and b chain-length of three BLFL-producing strains with different alcohol formation modules, BO33A-C (Table 2). SeAAR, MmCAR, and MaMaqu2507 are reductases from S. elongatus, M. marinum, and M. aquaeolei VT8, respectively. c, d Effect of alcohol dehydrogenase overexpression on BLFL production. Alcohol profiles of strain BO33A (without adh overexpression) are compared with those from strain BO33E1 (expressing adhA from L. lactis), BO33E2 (expressing yqhD from E. coli), and BO33E3 (expressing yjgB from E. coli). All cultures were supplemented with 1 g/L 4-methyl-2-oxopentanoic acid. Cells were cultivated and induced as described in “Methods” section