Table 1 Plasmids used in this research
From: Modular pathway engineering for the microbial production of branched-chain fatty alcohols
Plasmids | Replication ori | Overexpressed operon | Resistance | References |
|---|---|---|---|---|
pSa-PecfabH- Sa fabH | SC101 | PecfabH-Sa fabH (S. aureus) | AmpR | [28] |
pB5k-aar-lplA | pBBR1 | PlacUV5-aar (S. elongatus)-lplA (E. coli) | KanR | This study |
pB5k-sfp-car | pBBR1 | PlacUV5-sfp(B. subtilis)-car (M. marinum) | KanR | This study |
pE8c-tesA-lplA | ColE1 | PBAD-tesA (E. coli)-lplA (E. coli) | CmR | This study |
pB5k-maqu2507-lplA | pBBR1 | PlacUV5-maqu2507 (M. aquaeolei VT8)-lplA (E. coli) | KanR | This study |
pE5c-tesA-fadD | ColE1 | PlacUV5-tesA (E. coli)-fadD (E. coli) | CmR | [48] |
pB5k-maqu2220 | pBBR1 | PlacUV5-maqu2220 (M. aquaeolei VT8) | KanR | [48] |
pB5k-maqu2220-lplA | pBBR1 | PlacUV5-maqu2220 (M. aquaeolei VT8)-lplA (E. coli) | KanR | This study |
pB5k-adhA | pBBR1 | PlacUV5-adhA (L. lactis) | KanR | This study |
pB5k-yqhD | pBBR1 | PlacUV5-yqhD (E. coli) | KanR | This study |
pB5k-yjgB | pBBR1 | PlacUV5-yjgB (E. coli) | KanR | This study |
pA8c-aar-lplA | pA15a | PBAD-aar (S. elongatus)-lplA (E. coli) | CmR | This study |
pB2k-alsS-ilvCD-yjgB | pBBR1 | Ptet-alsS (B. subtilis)-ilvCD (E. coli)-yjgB(E. coli) | KanR | This study |
pE2c-aar-lplA-yjgB | ColE1 | Ptet-aar (S. elongatus)-lplA (E. coli)-yjgB (E. coli) | CmR | This study |
pA6k-alsS-ilvCD | pA15a | PLlacO-alsS (B. subtilis)-ilvCD (E. coli) | KanR | [43] |
pB2k-alsS-ilvCD | pBBR1 | Ptet-alsS (B. subtilis)-ilvCD (E. coli) | KanR | This study |
pB5c-aar-lplA-yjgB | pBBR1 | PlacUV5-aar (S. elongatus)-lplA (E. coli)-yjgB (E. coli) | CmR | This study |
pB5c-maqu2220-lplA | pBBR1 | PlacUV5-maqu2220 (M. aquaeolei VT8)-lplA (E. coli) | CmR | This study |
pA8c-leuA mut BCD | pA15a | PBAD-leuA mut BCD (E. coli) | CmR | This study |
pE8c-leuA mut BCD | ColE1 | PBAD-leuA mut BCD (E. coli) | CmR | This study |
pB5k-maqu2220-leuA mut BCD | pBBR1 | PlacUV5-maqu2220 (M. aquaeolei VT8)-leuA mut BCD (E. coli) | KanR | This study |
pA8c-lplA | pA15a | PBAD-lplA (E. coli) | CmR | This study |
pE8c-adhA | ColE1 | PBAD-adhA (L. lactis) | CmR | This study |
pE8c-yqhD | ColE1 | PBAD-yqhD (E. coli) | CmR | This study |
pE8c-yjgB | ColE1 | PBAD-yjgB (E. coli) | CmR | This study |
pE2s-alsS-ilvCD | ColE1 | Ptet-alsS (B. subtilis)-ilvCD (E. coli) | SpecR | This study |