Fig. 5
The hydrogen production from glucose compared by IAM1183 among all strains in serum bottle batch assays (n = 3). The yield of H2 generated through the formate pathway can be calculated by the following equation V h  = V e  + V a  − V f , where V h is the amount of hydrogen produced through the formate pathway, V e is the amount of ethanol, V a is the amount of acetate, and V f is the amount of formate. a Hydrogen evolved from IAM1183-CD and IAM1183-CDE. b Hydrogen evolved from IAM1183/R, IAM1183-CD/R, and IAM1183-CDE/R. c Hydrogen evolved from IAM1183/N, IAM1183-CD/N, and IAM1183-CDE/N. d Special illustration of hydrogen evolved via the NADH pathway