Table 1 Strains and plasmids used in this study
Strain or plasmid | Genotype and relevant characteristics | Source or literature |
|---|---|---|
Strains | ||
 IAM1183 | Wild type | IAM (Tokyo, Japan) |
 IAM1183/N | Carrying plasmid pET-28a-nadE | This study |
 IAM1183/R | Carrying plasmid pKK102-ryhB-cm | This study |
 IAM1183/NR | Carrying plasmid pET-28a-nadE and pKK102-ryhB-cm | This study |
 IAM1183-CD | ∆nuoC/∆nuoD | This study |
 IAM1183-CD/N | ∆nuoC/∆nuoD, carrying plasmid pET-28a-nadE | This study |
 IAM1183-CD/R | ∆nuoC/∆nuoD, carrying plasmid pKK102-ryhB-cm | This study |
 IAM1183-CD/RN | ∆nuoC/∆nuoD, carrying plasmid pET-28a-nadE and pKK102-ryhB-cm | This study |
 IAM1183-CDE | ∆nuoC/∆nuoD/∆nuoE | This study |
 IAM1183-CDE/N | ∆nuoC/∆nuoD/∆nuoE, carrying plasmid pET-28a-nadE | This study |
 IAM1183-CDE/R | ∆nuoC/∆nuoD/∆nuoE, carrying plasmid pKK102-ryhB-cm | This study |
 IAM1183-CD/RN | ∆nuoC/∆nuoD/∆nuoE, carrying plasmid pET-28a-nadE and pKK102-ryhB-cm | This study |
 E. coli DH5α | F-, φ 80dlacZ ΔM15, Δ (lacZYA -argF) U169, deoR, recA1, endA1, hsdR17 (rK-, mK +), phoA, supE44, λ-, thi -1, gyrA96, relA1 | TaRaKa |
 E. coli JM109 | recA1, endA1, gyrA96, thi-1, hsdR17 (rk-mk +), e14-(mcrA-) supE44, relA1, Δ(lac-proAB)/F’[traD36, proAB+, lacIq, lacZ ΔM15] | TaRaKa |
 K. pneumoniae nov. sp | Wild type | This study |
Plasmid | ||
 pCAS9 | repA101(Ts) kan Pcas-cas9 ParaB-Red lacIq Ptrc-sgRNA-pMB1 | [28] |
 PTargetF-cat | pMB1 aadA sgRNA-cat | [28] |
 PTargetF-nuoN20 | pMB1 aadA sgRNA-nuoN20 | This study |
 pET-28a | N-His, N-Thrombin, N-T7, C-His, Kanr | TaRaKa |
 pSTV28 | Cm r, with multiple coning sites | TaRaKa |
 pMD18-T Vector | TA Cloning vector, Amp r | TaRaKa |
 pMD18-T-nadE | Amp r, derived from pMD18-T inserted nadE | This study |
 pET-28a-nadE | nadE, Kan r derived from pET-28a | This study |
 pKK102-ryhB | RyhB, Amp r | [23] |
 pKK102-ryhB-cm | RyhB, Cm r derived pKK102-ryhB | This study |