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Table 1 Strains and plasmids used in this study

From: Impairment of NADH dehydrogenase and regulation of anaerobic metabolism by the small RNA RyhB and NadE for improved biohydrogen production in Enterobacter aerogenes

Strain or plasmid Genotype and relevant characteristics Source or literature
Strains
 IAM1183 Wild type IAM (Tokyo, Japan)
 IAM1183/N Carrying plasmid pET-28a-nadE This study
 IAM1183/R Carrying plasmid pKK102-ryhB-cm This study
 IAM1183/NR Carrying plasmid pET-28a-nadE and pKK102-ryhB-cm This study
 IAM1183-CD nuoC/∆nuoD This study
 IAM1183-CD/N nuoC/∆nuoD, carrying plasmid pET-28a-nadE This study
 IAM1183-CD/R nuoC/∆nuoD, carrying plasmid pKK102-ryhB-cm This study
 IAM1183-CD/RN nuoC/∆nuoD, carrying plasmid pET-28a-nadE and pKK102-ryhB-cm This study
 IAM1183-CDE nuoC/∆nuoD/∆nuoE This study
 IAM1183-CDE/N nuoC/∆nuoD/∆nuoE, carrying plasmid pET-28a-nadE This study
 IAM1183-CDE/R nuoC/∆nuoD/∆nuoE, carrying plasmid pKK102-ryhB-cm This study
 IAM1183-CD/RN nuoC/∆nuoD/∆nuoE, carrying plasmid pET-28a-nadE and pKK102-ryhB-cm This study
 E. coli DH5α F-, φ 80dlacZ ΔM15, Δ (lacZYA -argF) U169, deoR, recA1, endA1, hsdR17 (rK-, mK +), phoA, supE44, λ-, thi -1, gyrA96, relA1 TaRaKa
 E. coli JM109 recA1, endA1, gyrA96, thi-1, hsdR17 (rk-mk +), e14-(mcrA-) supE44, relA1, Δ(lac-proAB)/F’[traD36, proAB+, lacIq, lacZ ΔM15] TaRaKa
 K. pneumoniae nov. sp Wild type This study
Plasmid
 pCAS9 repA101(Ts) kan Pcas-cas9 ParaB-Red lacIq Ptrc-sgRNA-pMB1 [28]
 PTargetF-cat pMB1 aadA sgRNA-cat [28]
 PTargetF-nuoN20 pMB1 aadA sgRNA-nuoN20 This study
 pET-28a N-His, N-Thrombin, N-T7, C-His, Kanr TaRaKa
 pSTV28 Cm r, with multiple coning sites TaRaKa
 pMD18-T Vector TA Cloning vector, Amp r TaRaKa
 pMD18-T-nadE Amp r, derived from pMD18-T inserted nadE This study
 pET-28a-nadE nadE, Kan r derived from pET-28a This study
 pKK102-ryhB RyhB, Amp r [23]
 pKK102-ryhB-cm RyhB, Cm r derived pKK102-ryhB This study