Table 2 Primers used in the experiments
Primers | Oligonucleotide sequence (from 5′ to 3′) | PCR fragment | Source |
|---|---|---|---|
F-CDuh | CCCTGCAACTTCGGCCTGTCA | Upstream-homologous arm of nuoB | This study |
R-CDuh | TGATTCTCGTGCATTTAAATCTCGTCCGGTGT | This study | |
F-CDdh | ACCGGACGAGATTTAAATGCACGAGAATCAAC | Downstream-homologous arm of nuoE | This study |
R-CDdh | CTGCTCCAACAGGTCAGGAATCG | This study | |
F-CDEuh | AGCAAGAAGTCAATAAAAGCGT | Upstream-homologous arm of nuoB | This study |
R-CDEuh | ATCACTGTCTTCATTTAAATCTCGTCCGGTGT | This study | |
F-CDEdh | CGAGATTTAAATGAAGACAGTGATCCGCA | Downstream-homologous arm of nuoF | This study |
R-CDEdh | ACGGCCTTCAAGGGAGTTAATC | This study | |
F-nuoCD | TCCTAGGTATAATACTAGTGGCGAACTGCGCAATCGTTTTGGGTTTTAGAGCTAGAAATAGC | nuoCD-N20 | This study |
R-nuoCD | ACTAGTATTATACCTAGGACTGAGCTAGCTGTCAAG | This study | |
F-nadE | CCGGAATTCATGACGCTACAACAAGAGATT | nadE ORF fragment | This study |
R-nadE | CCCAAGCTTCTATTTTTTCCAGAAATCGTC | This study | |
F-p102-Chol | TGCACGGTGCACAGTGCCAAGC TTGCATGCCT | Chol ORF fragment | This study |
R-p102-Chol | CGGCATGACTCCCCGTCAGTATACACTCCGCTAGCGC | This study |