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Table 4 Analysis of consumed substrate and the production of anaerobic metabolites after 20 h of cultivation in a serum bottle for wild-type IAM1183 and mutants (n = 3)

From: Impairment of NADH dehydrogenase and regulation of anaerobic metabolism by the small RNA RyhB and NadE for improved biohydrogen production in Enterobacter aerogenes

End product

Strain

IAM1183

IAM1183/R

IAM1183/N

IAM1183-CD

IAM1183-CD/R

IAM1183-CD/N

IAM1183-CDE

IAM1183-CDE/R

IAM1183-CDE/N

Glucose consumed (mM)

57.42 ± 1.38

59.27 ± 0.69

60.62 ± 0.85

70.02 ± 0.66

68.13 ± 0.94

74.05 ± 0.43

69.02 ± 0.77

69.28 ± 0.49

71.09 ± 0.39

Residual sugar (mM)

4.66 ± 0.25

4.33 ± 0.12

4.09 ± 0.15

2,4 ± 0.12

2.74 ± 0.17

1.67 ± 0.08

2.58 ± 0.14

2.53 ± 0.09

2.2 ± 0.07

2,3-Butanediol (mM)

9.35 ± 1.56

11.86 ± 0.91

11.53 ± 0.78

11.73 ± 1.37

24.75 ± 0.42

13.79 ± 1.09

8.26 ± 0.17

11.96 ± 0.78

10.69 ± 0.26

Lactate (mM)

23.62 ± 0.46

44.15 ± 0.99

31.54 ± 0.96

18.78 ± 1.03

25.33 ± 0.81

22.31 ± 0.66

32.14 ± 0.29

36.61 ± 0.45

38.21 ± 0.36

Acetate (mM)

17.27 ± 0.32

10.36 ± 0.39

20.31 ± 0.65

27.96 ± 0.69

23.17 ± 0.55

30.52 ± 1.26

17.16 ± 0.31

15.27 ± 0.13

16.01 ± 0.63

Formate (mM)

2.24 ± 0.17

3.92 ± 0.77

2.83 ± 0.98

6.19 ± 0.76

5.98 ± 0.47

8.28 ± 1.62

3.67 ± 0.34

2.92 ± 0.19

1.63 ± 0.27

Succinate

1.86 ± 0.08

1.01 ± 0.11

2.31 ± 0.19

3.22 ± 0.17

3.02 ± 0.12

3.59 ± 0.36

3.79 ± 0.48

3.02 ± 0.12

3.86 ± 0.23

Ethanol (mM)

31.93 ± 1.2

13.85 ± 2.02

38.09 ± 0.98

38.69 ± 1.16

14.47 ± 1.32

40.87 ± 0.72

25.14 ± 0.26

23.49 ± 1.19

29.96 ± 0.77

Citric acid (mM)

1.68 ± 0.25

1.52 ± 0.49

1.43 ± 0.33

1.69 ± 0.37

1.51 ± 0.72

1.65 ± 0.96

1.57 ± 0.67

1.62 ± 0.22

1.64 ± 0.93

Acetoin (mM)

25.71 ± 1.11

19.21 ± 0.58

27.32 ± 0.64

20.99 ± 0.84

20.13 ± 0.74

25.02 ± 0.17

23.19 ± 0.29

26.81 ± 0.44

25.62 ± 0.51

H2 yield (mol/mol glucose)

1.47 ± 0.06

1.52 ± 0.13

1.56 ± 0.17

1.83 ± 0.09

1.67 ± 0.41

1.99 ± 0.22

2.14 ± 0.69

2.08 ± 0.12

2.28 ± 0.12