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Table 4 Analysis of consumed substrate and the production of anaerobic metabolites after 20 h of cultivation in a serum bottle for wild-type IAM1183 and mutants (n = 3)

From: Impairment of NADH dehydrogenase and regulation of anaerobic metabolism by the small RNA RyhB and NadE for improved biohydrogen production in Enterobacter aerogenes

End product Strain
IAM1183 IAM1183/R IAM1183/N IAM1183-CD IAM1183-CD/R IAM1183-CD/N IAM1183-CDE IAM1183-CDE/R IAM1183-CDE/N
Glucose consumed (mM) 57.42 ± 1.38 59.27 ± 0.69 60.62 ± 0.85 70.02 ± 0.66 68.13 ± 0.94 74.05 ± 0.43 69.02 ± 0.77 69.28 ± 0.49 71.09 ± 0.39
Residual sugar (mM) 4.66 ± 0.25 4.33 ± 0.12 4.09 ± 0.15 2,4 ± 0.12 2.74 ± 0.17 1.67 ± 0.08 2.58 ± 0.14 2.53 ± 0.09 2.2 ± 0.07
2,3-Butanediol (mM) 9.35 ± 1.56 11.86 ± 0.91 11.53 ± 0.78 11.73 ± 1.37 24.75 ± 0.42 13.79 ± 1.09 8.26 ± 0.17 11.96 ± 0.78 10.69 ± 0.26
Lactate (mM) 23.62 ± 0.46 44.15 ± 0.99 31.54 ± 0.96 18.78 ± 1.03 25.33 ± 0.81 22.31 ± 0.66 32.14 ± 0.29 36.61 ± 0.45 38.21 ± 0.36
Acetate (mM) 17.27 ± 0.32 10.36 ± 0.39 20.31 ± 0.65 27.96 ± 0.69 23.17 ± 0.55 30.52 ± 1.26 17.16 ± 0.31 15.27 ± 0.13 16.01 ± 0.63
Formate (mM) 2.24 ± 0.17 3.92 ± 0.77 2.83 ± 0.98 6.19 ± 0.76 5.98 ± 0.47 8.28 ± 1.62 3.67 ± 0.34 2.92 ± 0.19 1.63 ± 0.27
Succinate 1.86 ± 0.08 1.01 ± 0.11 2.31 ± 0.19 3.22 ± 0.17 3.02 ± 0.12 3.59 ± 0.36 3.79 ± 0.48 3.02 ± 0.12 3.86 ± 0.23
Ethanol (mM) 31.93 ± 1.2 13.85 ± 2.02 38.09 ± 0.98 38.69 ± 1.16 14.47 ± 1.32 40.87 ± 0.72 25.14 ± 0.26 23.49 ± 1.19 29.96 ± 0.77
Citric acid (mM) 1.68 ± 0.25 1.52 ± 0.49 1.43 ± 0.33 1.69 ± 0.37 1.51 ± 0.72 1.65 ± 0.96 1.57 ± 0.67 1.62 ± 0.22 1.64 ± 0.93
Acetoin (mM) 25.71 ± 1.11 19.21 ± 0.58 27.32 ± 0.64 20.99 ± 0.84 20.13 ± 0.74 25.02 ± 0.17 23.19 ± 0.29 26.81 ± 0.44 25.62 ± 0.51
H2 yield (mol/mol glucose) 1.47 ± 0.06 1.52 ± 0.13 1.56 ± 0.17 1.83 ± 0.09 1.67 ± 0.41 1.99 ± 0.22 2.14 ± 0.69 2.08 ± 0.12 2.28 ± 0.12