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Table 1 Strains and plasmids used in this work

From: Expression of adhA from different organisms in Clostridium thermocellum

Strain ID

Plasmid ID

Source of adhA on the plasmid

Source organism abbreviation

Sequence identity to Tsac AdhA

Source organism optimal growth temperature (°C)

GenBank accession number

LL1525

pCBcth1

Thermoanaerobacter mathranii

Tmat

86%

70–75 [17]

MG026506

LL1526

pCBcth2

Thermoanaerobacterium ethanolicus

Teth

88%

70 [18]

 MG026510

LL1527

pCBcth3

Clostridium botulinum

Cbot

62%

37 [19]

 MG026514

LL1528

pCBcth4

Thermococcus strain AN1

Ther

65%

75–80 [20]

 MG026513

LL1529

pCBcth7

Thermotoga petrophila

Tpet

60%

80 [21]

 MG026511

LL1530

pCBcth8

Methanocaldococcus bathoardescens

Mbat

57%

82 [22]

 MG026508

LL1531

pCBcth9

Marinitoga piezophila

Mpie

61%

65 [23]

 MG026515

LL1532

pCBcth12

Thermoanaerobacterium thermosaccharolyticum

Tthe

90%

68 [24]

 MG026507

LL1533

pCBcth13

Fervidobacterium nodosum

Fnod

67%

65–70 [25]

 MG026509

LL1534

pCBcth14

Caldicellulosiruptor saccharolyticus

Csac

76%

70 [26]

 MG026512

LL1535

pDGO144 [9]a

NA

NA

NA

55

 

LL1536

pCBcth17

Thermoanaerobacterium saccharolyticum

Tsac

100%

60 [27]

 MG026516

  1. NA not applicable
  2. aThe empty vector pDGO144 is also known as pCBcth15