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Table 1 Strains and plasmids used in this work

From: Expression of adhA from different organisms in Clostridium thermocellum

Strain ID Plasmid ID Source of adhA on the plasmid Source organism abbreviation Sequence identity to Tsac AdhA Source organism optimal growth temperature (°C) GenBank accession number
LL1525 pCBcth1 Thermoanaerobacter mathranii Tmat 86% 70–75 [17] MG026506
LL1526 pCBcth2 Thermoanaerobacterium ethanolicus Teth 88% 70 [18]  MG026510
LL1527 pCBcth3 Clostridium botulinum Cbot 62% 37 [19]  MG026514
LL1528 pCBcth4 Thermococcus strain AN1 Ther 65% 75–80 [20]  MG026513
LL1529 pCBcth7 Thermotoga petrophila Tpet 60% 80 [21]  MG026511
LL1530 pCBcth8 Methanocaldococcus bathoardescens Mbat 57% 82 [22]  MG026508
LL1531 pCBcth9 Marinitoga piezophila Mpie 61% 65 [23]  MG026515
LL1532 pCBcth12 Thermoanaerobacterium thermosaccharolyticum Tthe 90% 68 [24]  MG026507
LL1533 pCBcth13 Fervidobacterium nodosum Fnod 67% 65–70 [25]  MG026509
LL1534 pCBcth14 Caldicellulosiruptor saccharolyticus Csac 76% 70 [26]  MG026512
LL1535 pDGO144 [9]a NA NA NA 55  
LL1536 pCBcth17 Thermoanaerobacterium saccharolyticum Tsac 100% 60 [27]  MG026516
  1. NA not applicable
  2. aThe empty vector pDGO144 is also known as pCBcth15