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Table 2 Fermentation end products of C. thermocellum strains expressing different adhA genes

From: Expression of adhA from different organisms in Clostridium thermocellum

Strain ID Source of adhA Ethanol Acetate Formate Lactate Malate Ethanol yield (% maximum theoretical) Carbon balance (%)
mM mM mM mM mM
LL1527 Cbot 32.16 ± 0.57 6.73 ± 0.90 3.51 ± 0.43 0.25 ± 0.04 0.56 ± 0.40 59 71
LL1526 Teth 26.27 ± 1.00 9.48 ± 1.64 8.78 ± 1.85 0.33 ± 0.01 0.39 ± 0.12 49 65
LL1525 Tmat 24.33 ± 2.50 10.07 ± 1.47 9.59 ± 1.11 0.51 ± 0.21 0.44 ± 0.18 45 63
LL1529 Tpet 24.07 ± 0.68 12.81 ± 0.74 4.82 ± 0.91 0.87 ± 0.12 0.50 ± 0.08 45 68
LL1530 Mbat 23.23 ± 1.56 12.30 ± 1.56 7.75 ± 1.99 0.94 ± 0.19 0.55 ± 0.10 43 66
LL1536 Tsac 23.23 ± 0.62 9.76 ± 0.67 7.53 ± 0.43 0.83 ± 0.00 0.65 ± 0.39 43 61
LL1528 Ther 23.21 ± 0.58 10.58 ± 0.43 8.22 ± 0.41 0.71 ± 0.06 0.42 ± 0.01 42 63
LL1532 Tthe 21.09 ± 0.37 12.95 ± 1.04 8.43 ± 1.53 0.63 ± 0.06 0.51 ± 0.13 39 63
LL1534 Csac 20.29 ± 0.35 12.93 ± 0.64 7.56 ± 0.46 0.74 ± 0.14 0.45 ± 0.03 38 62
LL1531 Mpie 20.26 ± 0.60 15.27 ± 0.27 9.08 ± 0.72 0.54 ± 0.06 0.63 ± 0.07 38 65
LL1533 Fnod 19.65 ± 1.71 14.21 ± 0.57 7.14 ± 1.88 1.32 ± 0.52 0.85 ± 0.12 36 64
LL1535 NA 15.67 ± 0.22 16.52 ± 0.94 3.04 ± 0.47 3.01 ± 1.17 0.98 ± 0.11 29 64
  1. Data shown here were based on triplicate experiments. Cultures were grown in MTC-5 medium with 4.7 g/l (13.8 mM) initial cellobiose at 55 °C for 72 h. All cultures completely consumed all of the cellobiose initially present in the medium. Thiamphenicol was added at 6 μg/ml for plasmid maintenance. Error is given as one standard deviation, n = 3. Rows are ordered by ethanol yield in descending order