Expression of adhA from different organisms in Clostridium thermocellum

Table 2 Fermentation end products of C. thermocellum strains expressing different adhA genes

Strain ID	Source of adhA	Ethanol	Acetate	Formate	Lactate	Malate	Ethanol yield (% maximum theoretical)	Carbon balance (%)
Strain ID	Source of adhA	mM	mM	mM	mM	mM	Ethanol yield (% maximum theoretical)	Carbon balance (%)
LL1527	Cbot	32.16 ± 0.57	6.73 ± 0.90	3.51 ± 0.43	0.25 ± 0.04	0.56 ± 0.40	59	71
LL1526	Teth	26.27 ± 1.00	9.48 ± 1.64	8.78 ± 1.85	0.33 ± 0.01	0.39 ± 0.12	49	65
LL1525	Tmat	24.33 ± 2.50	10.07 ± 1.47	9.59 ± 1.11	0.51 ± 0.21	0.44 ± 0.18	45	63
LL1529	Tpet	24.07 ± 0.68	12.81 ± 0.74	4.82 ± 0.91	0.87 ± 0.12	0.50 ± 0.08	45	68
LL1530	Mbat	23.23 ± 1.56	12.30 ± 1.56	7.75 ± 1.99	0.94 ± 0.19	0.55 ± 0.10	43	66
LL1536	Tsac	23.23 ± 0.62	9.76 ± 0.67	7.53 ± 0.43	0.83 ± 0.00	0.65 ± 0.39	43	61
LL1528	Ther	23.21 ± 0.58	10.58 ± 0.43	8.22 ± 0.41	0.71 ± 0.06	0.42 ± 0.01	42	63
LL1532	Tthe	21.09 ± 0.37	12.95 ± 1.04	8.43 ± 1.53	0.63 ± 0.06	0.51 ± 0.13	39	63
LL1534	Csac	20.29 ± 0.35	12.93 ± 0.64	7.56 ± 0.46	0.74 ± 0.14	0.45 ± 0.03	38	62
LL1531	Mpie	20.26 ± 0.60	15.27 ± 0.27	9.08 ± 0.72	0.54 ± 0.06	0.63 ± 0.07	38	65
LL1533	Fnod	19.65 ± 1.71	14.21 ± 0.57	7.14 ± 1.88	1.32 ± 0.52	0.85 ± 0.12	36	64
LL1535	NA	15.67 ± 0.22	16.52 ± 0.94	3.04 ± 0.47	3.01 ± 1.17	0.98 ± 0.11	29	64

Data shown here were based on triplicate experiments. Cultures were grown in MTC-5 medium with 4.7 g/l (13.8 mM) initial cellobiose at 55 °C for 72 h. All cultures completely consumed all of the cellobiose initially present in the medium. Thiamphenicol was added at 6 μg/ml for plasmid maintenance. Error is given as one standard deviation, n = 3. Rows are ordered by ethanol yield in descending order

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