Fig. 3

Algal transformation and screening. Step 1: the pDb124-Gal4 BD-CIB1 vector was transformed into CC-849, and the algae were screened with zeocin. Step 2: the pDh124-VP16-CRY2-UAS-amiRNA vector was transformed into transgenic algae from step 1, and the algae were screened with hygromycin B. Red arrows in the picture indicate positive clones, i.e., transgenic algal strains containing the blue light-inducible exogenous gene expression system