Fig. 8

Impact of xylooligomers on commercial cocktail performance. a Hydrolysis of Avicel by Accellerase^® in the presence of xylooligomers and compost21_GH11 during 96-h time course. b HPLC analysis showing xylooligomer degradation by Accellerase^® and c Accellerase^® supplemented with compost21_GH11, as assessed by detector response in nanocoulombs (nC). Note that in Fig. 3c xylobiose concentration increases after 1 h as a result of fast degradation of xylooligomers with degree of polymerization (DP) ≥ 4 by compost21_GH11. Higher DP xylooligomers have a higher inhibitory effect, thus its rapid degradation seems beneficial. The commercial cocktail used was able to degrade the added xylooligomers within 24 h. In consequence, after 96 h, reactions with and without compost21_GH11 achieved about the same glucose yields. However, at initial stages, the addition of compost21_GH11 strongly improved glucose production rate