Table 1 Purification yield of native and recombinant BGL-2 isoforms
Step | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Yield (%) |
|---|---|---|---|---|
BGL-2 | ||||
Crude extracts | 53.4 | 1039.7 | 19.5 | 100 |
HiTrap Capto Adhere | 15.8 | 795.6 | 50.4 | 76.5 |
Mono Q 5/50 | 7.6 | 596.4 | 78.5 | 57.3 |
Superdex 75 HR 10/30 | 0.8 | 66.1 | 82.6 | 6.3 |
BGL-2* | ||||
Crude extracts | 25.1 | 300.1 | 11.9 | 100 |
HiTrap Capto Adhere | 7.64 | 126.5 | 16.5 | 42.2 |
BGL-2T* | ||||
Crude extracts | 19.9 | 436.1 | 21.8 | 100 |
HiTrap Capto Adhere | 3.6 | 128.8 | 35.4 | 29.5 |