A novel, highly efficient β-glucosidase with a cellulose-binding domain: characterization and properties of native and recombinant proteins

Table 2 Kinetic parameters of all BGL-2 isoforms against different substrates

Substrate	K _m (mM)			k_cat (s⁻¹)			k_cat/K _m (mM⁻¹ s⁻¹)
Substrate	BGL-2	BGL-2*	BGL-2T*	BGL-2	BGL-2*	BGL-2T*	BGL-2	BGL-2*	BGL-2T*
pNPG	0.41 ± 0.02	0.19 ± 0.01	0.34 ± 0.02	485	444	874	1167	2243	2563
oNPG	0.86 ± 0.04	1.48 ± 0.02	0.67 ± 0.04	174	59	217	201	39	323
Cellobiose	1.21 ± 0.04	1.11 ± 0.02	0.91 ± 0.05	303	630	569	249	567	619
Cellotriose	1.37 ± 0.05	1.87 ± 0.13	1.42 ± 0.06	272	617	716	198	329	502
Cellotetraose	1.68 ± 0.04	0.92 ± 0.01	0.49 ± 0.03	313	580	402	185	629	813
Cellopentaose	1.28 ± 0.02	0.71 ± 0.03	0.68 ± 0.01	309	671	482	239	936	701
Cellohexaose	1.01 ± 0.04	0.51 ± 0.01	0.49 ± 0.03	313	405	359	307	794	720

ISSN: 2731-3654