Table 3 Comparison of cata lytic efficiencies against cellooligosaccharides (C2–C6) of fungal β-glucosidases
Organism and reference | Enzyme | Cellobiose | Cellotriose | Cellotetraose | Cellopentaose | Cellohexaose | ||||
|---|---|---|---|---|---|---|---|---|---|---|
K cat /K m | K m | V max | K m | V max | K m | V max | K m | V max | ||
Native BGLs | ||||||||||
Trichoderma reesei [50] | Cel3A | 37.3 | 0.2 | 38.0 | 41.0 | |||||
Cel3B | 21.6 | 0.3 | 36.0 | 36.0 | ||||||
Cel1A | 70.6 | 1.1 | 18.0 | 2.6 | ||||||
Metagenome from compost [51] | Td2f2 | 1.6 | 3.1 | 10.1 | 1.53 | 7.8 | 8.4 | 7.9 | ||
T. thermosaccharolyticum [52] | 13.3 | |||||||||
Penicillium purpurogenum [40] | 111.0 | 96.0 | 68.4 | |||||||
BGLs expressed in P. pastoris | ||||||||||
Aspergillus fumigatus [29] | rBgl3 | 52.1 | ||||||||
Penicillium funiculosum [32] | rBgl4 | 3610.4 | 59.4 | 39.3 | 32.7 | 23.70 | ||||
Neurospora crassa [33] | BGL2 | 12.3 | 9.4 | |||||||
Myceliophthora thermophila [30] | MtBgl3a | 17.4 | ||||||||
BGLs of this work | ||||||||||
T. amestolkiae | BGL-2 | 249.1 | 1.37 | 167.8 | 1.69 | 193.1 | 1.2 | 190.7 | 1.0 | 193.2 |
BGL-2* | 567.1 | 1.88 | 363.2 | 0.92 | 341.5 | 0.7 | 394.8 | 0.5 | 238.7 | |
BGL-2T | 619.1 | 1.43 | 532.2 | 0.50 | 298.9 | 0.6 | 358.1 | 0.5 | 266.9 | |