Fig. 4

Prevalence of bacterial and archaeal strains within different biogas-producing microbial communities as determined by the fragment recruitment approach. Metagenome sequences derived from the microbial communities of three mesophilic (BGP1-3) and one thermophilic biogas plants (BGP4) described previously [41] were mapped on the genome sequences of the 22 strains analyzed in this study, the four MAGs described previously [41], and 46 publicly available genomes obtained from the RefSeq database [44]. Results for the 25 most abundant organisms are shown in the upper part of the figure. The prevalence of the remaining eight isolates of this study, representing non-abundant organisms, is shown in the lower part of the figure. The x-axis represents the number of GPMs (genomes per million; analogous to TPM = transcripts Per Million), and the y-axis shows the analyzed organisms. Isolates investigated within this study are shown in red, genome bins obtained from a previous study [41] in blue, and genomes obtained from the RefSeq database are visualized in black