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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Quantitative in vivo phosphoproteomics reveals reversible signaling processes during nitrogen starvation and recovery in the biofuel model organism Chlamydomonas reinhardtii

Fig. 5

a Identification of regulated signaling pathways. a Motif-X analysis was done with significantly changed phosphopeptides in the one-way ANOVA. P value threshold was set to 0.0000001 and at least 10 occurrences were required. Chlamydomonas proteome was used as background proteome. b Protein–protein interaction network of phosphoproteins kinases present in both ANOVA and/or in common proteins datasets. Interaction networks were created using the STRING database for known and predicted protein–protein interactions [setting: high confidence (0.7); http://string-db.org/]. The list of protein sequences is provided in Additional file 2: Table S9. c Changes in phosphorylation of identified kinases and phosphatases. Phosphosites belonging to Kinases and Phosphatases present in the protein–protein interaction network in b and/or discussed in the text. In the protein–protein interaction network, DYRKP is DYRK2; NKK1 is EDP07608; and BSU1 is PKL1. Phosphopeptides are depicted in color while protein level is in black. Data represents Z-transformed normalized abundances of protein and phosphopeptides (n = 3 for phosphopeptides and n = 4 for protein level), Phosphosites followed by *are significant (Additional file 2: Table S2). Protein levels are based on data obtained previously [7]

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