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Table 2 Parameters fitted to our empirical data

From: An accurate description of Aspergillus niger organic acid batch fermentation through dynamic metabolic modelling

Parameter

Description

Value

v Pe,max (mmol gDW−1 h−1)

External phosphate maximum input ratea

0.08

KPe (mM)

External phosphate Michaelis constant

0.0333

v P,max (mmol gDW−1 h−1)

Internal phosphate maximum input rate

0.0008

K P (mM)

Internal phosphate Michaelis constant

0.0833

v G1 (mmol gDW−1 h−1)

External glucose passive uptake rate

0.00031419 × [GLC]b

v X1 (mmol gDW−1 h−1)

External xylose passive uptake rate

0.00033 × [XYL]c

v X2,max (mmol gDW−1 h−1)

External xylose high-affinity transport maximum rate

0.2

K X2 (mM)

External xylose high-affinity transport Michaelis constant

3.33

v X3,max (mmol gDW−1 h−1)

External xylose low-affinity transport maximum rate

2.5

K X3 (mM)

External xylose low-affinity transport Michaelis constant

3.33

[GOX] (mg gDW−1)

Concentration of external glucose oxidase enzyme

0.1

v CIT (mmol gDW−1 h−1)

Citric acid output rate constraintd

0.12

v OXAL (mmol gDW−1 h−1)

Oxalic acid output rate constraint

0.01

  1. aExternal phosphate input rate changed 8 h after the dFBA start time to 0.015 mmol gDW−1 h−1 if initial pH 2 or 0.004 mmol gDW−1 h−1 if initial pH 7
  2. b[GLC] is concentration of external glucose in mM
  3. c[XYL] is concentration of external xylose in mM
  4. dCitric acid output rate constraint changed to 0.016 mmol gDW−1 h−1 if initial pH above 2