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Table 2 Parameters fitted to our empirical data

From: An accurate description of Aspergillus niger organic acid batch fermentation through dynamic metabolic modelling

Parameter Description Value
v Pe,max (mmol gDW−1 h−1) External phosphate maximum input ratea 0.08
KPe (mM) External phosphate Michaelis constant 0.0333
v P,max (mmol gDW−1 h−1) Internal phosphate maximum input rate 0.0008
K P (mM) Internal phosphate Michaelis constant 0.0833
v G1 (mmol gDW−1 h−1) External glucose passive uptake rate 0.00031419 × [GLC]b
v X1 (mmol gDW−1 h−1) External xylose passive uptake rate 0.00033 × [XYL]c
v X2,max (mmol gDW−1 h−1) External xylose high-affinity transport maximum rate 0.2
K X2 (mM) External xylose high-affinity transport Michaelis constant 3.33
v X3,max (mmol gDW−1 h−1) External xylose low-affinity transport maximum rate 2.5
K X3 (mM) External xylose low-affinity transport Michaelis constant 3.33
[GOX] (mg gDW−1) Concentration of external glucose oxidase enzyme 0.1
v CIT (mmol gDW−1 h−1) Citric acid output rate constraintd 0.12
v OXAL (mmol gDW−1 h−1) Oxalic acid output rate constraint 0.01
  1. aExternal phosphate input rate changed 8 h after the dFBA start time to 0.015 mmol gDW−1 h−1 if initial pH 2 or 0.004 mmol gDW−1 h−1 if initial pH 7
  2. b[GLC] is concentration of external glucose in mM
  3. c[XYL] is concentration of external xylose in mM
  4. dCitric acid output rate constraint changed to 0.016 mmol gDW−1 h−1 if initial pH above 2