Fig. 3

Lignin, cellulose, xylan in corn stover cell wall shown in individual 3D concentration distribution (left) and overlay of their isosurfaces (right) by reconstruction of stimulated Raman scattering microscopy sectioning scans. Lignin is more concentrated at cell corner and compound middle lamella, while cellulose and xylan are more abundant in secondary wall. Raman frequencies used for stimulated Raman scattering microscopy: lignin—1600 cm⁻¹, cellulose—1100 cm⁻¹, and xylan—1471 cm⁻¹. CC cell corner; CML compound middle lamellae; SW secondary wall