Table 1 Adenylate energy charge (AEC) improvements observed through protocol development
Date | AEC | NADH (µM) | ATP (µM) | Protocol improvements from previous |
|---|---|---|---|---|
1 | 0.411 ± 0.017 | 0 | 0.246 ± 0.031 | Ethanol-based solvent, aggressive sonication protocol, extraction temperatures reached ~ 50 °C |
2 | 0.804 ± 0.009 | 0.123 ± 0.006 | 2.31 ± 0.13 | Fast-filtering extraction and aqueous/organic extraction solvent, adapted from [32] with modifications |
3 | 0.91 ± 0.01 | 1.17 ± 0.06 | 3.61 ± 0.02 | Further improved handling, removed formic acid from extraction solvent |