Species/strain | Genotype | Fermentation capabilities | Metabolite concentrations (μmol/g CDW) | NADH/NAD+ | NADPH/NADP+ | Adenylate energy charge | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
AMP | ADP | ATP | NAD | NADH | NADP | NADPH | ||||||
Clostridium thermocellum | ||||||||||||
LL345 | Δhpt | Wildtype | 0.12 ± 0.047 | 0.64 ± 0.23 | 4.22 ± 1.1 | 1.26 ± 0.4 | 0.05 ± 0.01 | 0.15 ± 0.03 | 0.17 ± 0.07 | 0.04 | 1.13 | 0.91 |
LL1111 | Δhpt ΔadhE | < 5% of wt ethanol | 0.53 ± 0.14 | 1.87 ± 0.54 | 5.64 ± 1.14 | 1.83 ± 0.42 | 0.48 ± 0.23 | 0.49 ± 0.04 | 0.71 ± 0.15 | 0.26 | 1.45 | 0.82 |
Thermoanaerobacterium saccharolyticum | ||||||||||||
LL1025 | Wildtype | Wildtype | 0.16 ± 0.078 | 0.88 ± 0.21 | 4.4 ± 1.26 | 2.41 ± 0.51 | 0.06 ± 0.01 | 0.52 ± 0.1 | 0.09 ± 0.02 | 0.02 | 0.17 | 0.89 |