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Table 2 Varying adenylate charge ratios observed across species highlights the need to develop protocols specific for each species/strain

From: Targeted redox and energy cofactor metabolomics in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum

Species/strain

Genotype

Fermentation capabilities

Metabolite concentrations (μmol/g CDW)

NADH/NAD+

NADPH/NADP+

Adenylate energy charge

AMP

ADP

ATP

NAD

NADH

NADP

NADPH

Clostridium thermocellum

LL345

Δhpt

Wildtype

0.12 ± 0.047

0.64 ± 0.23

4.22 ± 1.1

1.26 ± 0.4

0.05 ± 0.01

0.15 ± 0.03

0.17 ± 0.07

0.04

1.13

0.91

LL1111

Δhpt ΔadhE

< 5% of wt ethanol

0.53 ± 0.14

1.87 ± 0.54

5.64 ± 1.14

1.83 ± 0.42

0.48 ± 0.23

0.49 ± 0.04

0.71 ± 0.15

0.26

1.45

0.82

Thermoanaerobacterium saccharolyticum

LL1025

Wildtype

Wildtype

0.16 ± 0.078

0.88 ± 0.21

4.4 ± 1.26

2.41 ± 0.51

0.06 ± 0.01

0.52 ± 0.1

0.09 ± 0.02

0.02

0.17

0.89