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Table 2 Varying adenylate charge ratios observed across species highlights the need to develop protocols specific for each species/strain

From: Targeted redox and energy cofactor metabolomics in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum

Species/strain Genotype Fermentation capabilities Metabolite concentrations (μmol/g CDW) NADH/NAD+ NADPH/NADP+ Adenylate energy charge
AMP ADP ATP NAD NADH NADP NADPH
Clostridium thermocellum
LL345 Δhpt Wildtype 0.12 ± 0.047 0.64 ± 0.23 4.22 ± 1.1 1.26 ± 0.4 0.05 ± 0.01 0.15 ± 0.03 0.17 ± 0.07 0.04 1.13 0.91
LL1111 Δhpt ΔadhE < 5% of wt ethanol 0.53 ± 0.14 1.87 ± 0.54 5.64 ± 1.14 1.83 ± 0.42 0.48 ± 0.23 0.49 ± 0.04 0.71 ± 0.15 0.26 1.45 0.82
Thermoanaerobacterium saccharolyticum
LL1025 Wildtype Wildtype 0.16 ± 0.078 0.88 ± 0.21 4.4 ± 1.26 2.41 ± 0.51 0.06 ± 0.01 0.52 ± 0.1 0.09 ± 0.02 0.02 0.17 0.89