Fig. 4
a Measured EPR spectra of the BsCel5A-RtCBM11Y151R1 in solution in the absence and in the presence of increasing concentrations of β-glucan. The dash lines indicate the spectral lines in BsCel5A-RtCBM11Y151R1 spectra that are indicative of the immobilized protein population. b The Ms parameter from spectra of the BsCel5A-CBM11Y151R1 as a function of β-glucan concentration. The light gray line shows the fitted curve which yields a Ka value of 0.22 mg/mL