Skip to main content

Advertisement

Table 1 Kinetic parameters for β-glucan and CMC hydrolysis by cysteine mutants BsCel5A-CBM3 and BsCel5A-CBM11 enzymes

From: Lignocellulose binding of a Cel5A-RtCBM11 chimera with enhanced β-glucanase activity monitored by electron paramagnetic resonance

Enzyme Substrate V max (U mg−1) K 0.5 (mg mL−1) K cat (s−1) K cat/K 0.5 (mL mg−1 s−1)
BsCel5A-CBM3 β-glucan 498.1 ± 20.1 2.88 ± 0.05 472.34 ± 20.1 164.0
CMC 218.7 ± 10.4 6.97 ± 0.15 199.70 ± 10.4 28.65
BsCel5A-RtCBM11 β-glucan 985.7 ± 40.2 2.66 ± 0.04 934.72 ± 40.2 351.4
CMC 251.5 ± 9.6 6.98 ± 0.22 238.49 ± 9.6 34.16