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Table 1 Kinetic parameters for β-glucan and CMC hydrolysis by cysteine mutants BsCel5A-CBM3 and BsCel5A-CBM11 enzymes

From: Lignocellulose binding of a Cel5A-RtCBM11 chimera with enhanced β-glucanase activity monitored by electron paramagnetic resonance

Enzyme

Substrate

V max (U mg−1)

K 0.5 (mg mL−1)

K cat (s−1)

K cat/K 0.5 (mL mg−1 s−1)

BsCel5A-CBM3

β-glucan

498.1 ± 20.1

2.88 ± 0.05

472.34 ± 20.1

164.0

CMC

218.7 ± 10.4

6.97 ± 0.15

199.70 ± 10.4

28.65

BsCel5A-RtCBM11

β-glucan

985.7 ± 40.2

2.66 ± 0.04

934.72 ± 40.2

351.4

CMC

251.5 ± 9.6

6.98 ± 0.22

238.49 ± 9.6

34.16