Fig. 3

Deletion of PoxCxrA abolished the utilization of cellulose by P. oxalicum. a Growth of ΔPoxCxrA and ΔPoxKu70 on Avicel for 4 days after a shift from glucose; medium without inoculant was used as the blank control. (b–f) FPase, CMCase, pNPCase, pNPGase, and xylanase activities, respectively, of crude enzymes from ΔPoxCxrA and ΔPoxKu70 after a shift from glucose to Avicel. Crude enzymes were produced by fungal strains cultured on 2% Avicel as the sole carbon source. Enzyme activities were measured 1, 2, 3, 4, and 5 days after the shift. Data are the means of three biological replicates. FPase filter-paper cellulase, CMCase carboxymethylcellulase, pNPCase p-nitrophenyl-β-cellobiosidase, pNPGase p-nitrophenyl-β-glucopyranosidase