Fig. 2
Shaking flask cultivations of C. glutamicum wild-type under various growth conditions. a Reference cultivations with supplementation of 5 g YE L−1 as sole carbon source (circles) as well as experiments with 3.5% (v/v) and rel. 3.5% (v/v) PW that was clarified (diamonds) or with 1 h heat treated (HT) PW without YE (triangles). b Growth on differently HT PW [0.5 h (crosses), 1 h (triangles), and 1.5 h (diamonds)] as well as 1 h HT PW with 1 mM reduced glutathione (GSH). c Course of acetate and acetol concentrations during growth on 1 h HT PW. d Investigation of the use of acetol as carbon source. Sole adding of acetol (diamonds) and YE (circles) is shown. The acetol consumption (open triangles) refers to the experiment of acetol and YE supplementation (triangles). Error bars represent the SD of ≥ 3 independent cultivations and measurements