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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Valorization of pyrolysis water: a biorefinery side stream, for 1,2-propanediol production with engineered Corynebacterium glutamicum

Fig. 3

1,2-PDO production using pyrolysis water (PW) with C. glutamicum PDO1 (a; wild-type + pJULgldA) and C. glutamicum PDO2 (b; Δpqo ΔaceE ΔldhA Δmdh + pJULgldA). Cultivations were performed in shaking flasks (1; CGXII* medium) and bioreactors (2; CGXII** medium) and were supplemented with PW (clarified and exposed to 1 h heat treatment at 80 °C) and yeast extract (YE). Depicted are the concentrations of cell dry weight (CDW) in g L−1 and acetate, acetol, and 1,2-PDO in mM over the process time. PW and YE were fed intermittently after the initial batch phase at indicated time points in the aerobic fermentation (a2; F1, F2) and two-phase aerobic/microaerobic processes (b2; F1). A gradually increasing oxygen deprivation is indicated by a triangle in the feed phase (F1). The product yields (Y P/S) are shown in mol 1,2-PDO per mol acetol (c). Error bars represent SD of triplicate experiments

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