Valorization of pyrolysis water: a biorefinery side stream, for 1,2-propanediol production with engineered Corynebacterium glutamicum

Table 3 Parameters during shaking flask cultivations of C. glutamicum wild-type using pyrolysis water as substrate

		1 h HT PW + YE	1.5 h HT PW + YE	1 h HT PW + GSH	Acetol + YE
µ	(h⁻¹)	0.31 ± 0.06	0.36 ± 0.04	0.18 ± 0.02	0.03 ± 0.01^a
q ^max_S	(mmol_acetate g ⁻¹_CDW h⁻¹)	10.0 ± 1.2	11.1 ± 2.2	9.4 ± 0.8	–
q ^max_S	(mmol_acetol g ⁻¹_CDW h⁻¹)	1.3 ± 0.3	1.0 ± 0.1	1.1 ± 0.2	3.6 ± 0.7
Y _X/S*	(g_CDW g ⁻¹_acetate )	0.83 ± 0.05	0.86 ± 0.08	0.44 ± 0.01	–
Y _X/S*	(g_CDW g ⁻¹_acetol )	–	–	–	0.24 ± 0.09^a

Growth rate (µ), maximum differential biomass-specific substrate uptake rates (q ^max_S ) and the apparent biomass substrate yields (Y _X/S*) in respective shaking flask experiments with 1 h and 1.5 h HT PW containing either 5 g yeast extract (YE) L⁻¹ or 1 mM reduced glutathione (GSH) as well as 5 g acetol L⁻¹ with 5 g YE L⁻¹. Error bars depict the SD of ≥ 3 independent experiments
^aCalculated for the second growth phase (cf. Fig. 2d)

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