Fig. 1

Fractionation of culture filtrate produced by strain KSM-F532 and the saccharification efficiency of the fractions. a Elution profile of extracellular proteins from KSM-F532 culture supernatant by gel-filtration chromatography. b SDS-PAGE of each fraction; 8 µL of each fraction was loaded (fractions 17–27 shown). Lane M: Precision Plus Protein Unstained Standard (Bio-Rad). c Saccharification of alkaline-pretreated bagasse using a mixture of enzyme preparation JN11H (1.0 mg/g-biomass) and fractionated enzymes (0.1 mg/g-biomass) produced by KSM-F532. Saccharification experiments were carried out at pH 5.0, 50 °C with 5% (w/v) alkaline-pretreated bagasse. Black bars indicate glucose yield and gray bars indicate xylose yield. The glucose and xylose yields were calculated from the mass of glucose and xylose released after 72 h. The glucose and xylose yields represent the mean of triplicate experiments. Error bars indicate standard deviations. – no fraction was added; W whole culture filtrate of KSM-F532 before fractionation was added