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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: A novel GH10 xylanase from Penicillium sp. accelerates saccharification of alkaline-pretreated bagasse by an enzyme from recombinant Trichoderma reesei expressing Aspergillus β-glucosidase

Fig. 4

Enzymatic characteristics of purified PspXyn10. a Effect of temperature on the xylanase activity of purified PspXyn10. The enzyme reaction was carried out in 50 mM sodium acetate buffer (pH 5.0) for 5 min for 1% (w/v) beechwood xylan with 1 mg/L enzyme at different temperatures. b Effect of temperature on the stability of purified PspXyn10. The enzyme (10 mg/L) was incubated in 50 mM sodium acetate buffer (pH 5.0) at different temperatures for 10 min, and then the residual activity for hydrolysis of beechwood xylan was quantified. The enzyme reaction was carried out in 50 mM sodium acetate buffer (pH 5.0) for 1% (w/v) beechwood xylan with 1 mg/L enzyme at 50 °C for 5 min. c Effect of pH on the xylanase activity of purified PspXyn10. The enzyme reaction was carried out in 50 mM sodium acetate buffer (pH 3.0–5.5) or 50 mM sodium phosphate buffer (pH 6.0–7.0) for 5 min for 1% (w/v) beechwood xylan with 1 mg/L enzyme at 50 °C. The values of relative activities are the means of three replicate. Error bars indicate standard deviations

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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