Table 3 Plasmids, PCR templates, and primers used for plasmid construction
Plasmid | Template | Forward primer | Reverse primer |
|---|---|---|---|
pUC-cbh1 | gDNA of PC-3-7 | SwaI cbh1 F | SwaI cbh1 R |
pUC118 | SwaI pUC F | SwaI pUC R | |
pUC-cbh1-amdS | pUC-cbh1 | cbh1-amdS F | cbh1-amdS R |
pUC-amdS | amdS F | amdS R | |
pUC-Pcbh1-pspxyn10-amdS | pUC-cbh1-amdS | Tcbh1 F | Pcbh1 R |
cDNA of KSM-F532 | Pcbh1-pspxyn F | pspxyn-Tcbh1 R | |
pUC-xyn2 | gDNA of PC-3-7 | SwaI xyn2 F | SwaI xyn2 R |
pUC118 | SwaI pUC F | SwaI pUC R | |
pUC-xyn2-pyr4 | pUC-xyn2 | xyn2-pyr4 F | xyn2-pyr4 R |
gDNA of PC-3-7 | pyr4 F | pyr4 R | |
pUC-Pxyn2-pspxyn10-pyr4 | pUC-xyn2-pyr4 | Txyn2 F | Pxyn2 R |
cDNA of KSM-F532 | Pxyn2-pspxyn F | pspxyn-Txyn2 R |