Skip to main content

Advertisement

Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Defined tetra-allelic gene disruption of the 4-coumarate:coenzyme A ligase 1 (Pv4CL1) gene by CRISPR/Cas9 in switchgrass results in lignin reduction and improved sugar release

Fig. 3

Results of 2nd PCR/sequencing showing mutations in Pv4CL1. pv4cl1-25 had four different mutation patterns, − 29/+ 18, − 27, − 32, and − 213; pv4cl1-26 had two different mutation patterns, − 27 and − 32/+ 16; pv4cl1-28 had two different mutation patterns, − 27 and − 1; pv4cl1-29 had three different mutation patterns, − 44, − 22, and − 14/+ 7 in Pv4CL1. Blue letters: the target site of Pv4CL1; dark red letters: SNPs; bright red letters: insertion sequences

Back to article page