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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Green route to synthesis of valuable chemical 6-hydroxynicotine from nicotine in tobacco wastes using genetically engineered Agrobacterium tumefaciens S33

Fig. 2

Purification and properties of the Hno from A. tumefaciens S33. a The wild-type Hno purified from A. tumefaciens S33. M, markers; 1, DEAE Sepharose Fast Flow; lane 2, Q Sepharose. b The recombinant His-tagged Hno. Lane 1, cell extracts of the recombinant E. coli BL21 (DE3); lane 2, His Trap HP; lane 3, DEAE Sepharose Fast Flow; M, markers. c UV–visible absorption spectrum of 0.6 mg mL−1 purified recombinant His-tagged Hno in 50 mM sodium phosphate buffer (pH 7.0). d Determination of the kinetic constants from the 6-hydroxynicotine oxidation. e UV–visible absorption spectra of the substrate 6-hydroxynicotine (solid line) and the products of 6-hydroxynicotine oxidation catalyzed by purified recombinant Hno (dashed line)

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