Green route to synthesis of valuable chemical 6-hydroxynicotine from nicotine in tobacco wastes using genetically engineered Agrobacterium tumefaciens S33

Table 2 Strains and plasmids used in this study

Strain or plasmid	Description	Source
Strains
E. coli
BL21 (DE3)	F⁻ ompT hsdS(r ⁻_B m ⁻_B ) gal dcm lacY1(DE3)	Novagen
A. tumefaciens
S33	Wild-type, nicotine degrader; G⁻	[4]
S33-∆hno	Gm^r; hno mutant of strain S33	This study
S33-∆hno-C	Gm^r; strain S33-∆hno containing pBBR-hno	This study
Plasmid
pETDuet-1	Ap^r; expression vector	Novagen
pETDuet-1-hno	Ap^r; pETDuet-1 containing hno gene	This study
pJQ200SK	Gm^r; mob⁺ orip 15A, lacZα⁺ sac B; suicide plasmid	[39]
pJQ-∆hno	Gm^r; hno were disrupted and inserted into pJQ200SK	This study
pRK2013	Km^r; helper plasmid for conjugation	Clontech
pBBR1MCS-5	Gm^r; broad-host-range cloning vector	[40]
pBBR-hno	Gm^r; hno inserted into pBBR1-MCS5	This study

ISSN: 1754-6834