Table 2 Strains and plasmids used in this study

Strains

 E. coli

  BL21 (DE3)

F⁻ ompT hsdS(r ⁻_B m ⁻_B ) gal dcm lacY1(DE3)

Novagen

 A. tumefaciens

  S33

Wild-type, nicotine degrader; G⁻

[4]

  S33-∆hno

Gm^r; hno mutant of strain S33

This study

  S33-∆hno-C

Gm^r; strain S33-∆hno containing pBBR-hno

This study

Plasmid

 pETDuet-1

Ap^r; expression vector

Novagen

 pETDuet-1-hno

Ap^r; pETDuet-1 containing hno gene

This study

 pJQ200SK

Gm^r; mob⁺ orip 15A, lacZα⁺ sac B; suicide plasmid

[39]

 pJQ-∆hno

Gm^r; hno were disrupted and inserted into pJQ200SK

This study

 pRK2013

Km^r; helper plasmid for conjugation

Clontech

 pBBR1MCS-5

Gm^r; broad-host-range cloning vector

[40]

 pBBR-hno

Gm^r; hno inserted into pBBR1-MCS5

This study