Strains
|
E. coli
|
BL21 (DE3)
|
F−
ompT hsdS(r
−B
m
−B
) gal dcm lacY1(DE3)
|
Novagen
|
A. tumefaciens
|
S33
|
Wild-type, nicotine degrader; G−
|
[4]
|
S33-∆hno
|
Gmr; hno mutant of strain S33
|
This study
|
S33-∆hno-C
|
Gmr; strain S33-∆hno containing pBBR-hno
|
This study
|
Plasmid
|
pETDuet-1
|
Apr; expression vector
|
Novagen
|
pETDuet-1-hno
|
Apr; pETDuet-1 containing hno gene
|
This study
|
pJQ200SK
|
Gmr; mob+
orip 15A, lacZα+ sac B; suicide plasmid
|
[39]
|
pJQ-∆hno
|
Gmr; hno were disrupted and inserted into pJQ200SK
|
This study
|
pRK2013
|
Kmr; helper plasmid for conjugation
|
Clontech
|
pBBR1MCS-5
|
Gmr; broad-host-range cloning vector
|
[40]
|
pBBR-hno
|
Gmr; hno inserted into pBBR1-MCS5
|
This study
|