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Table 1 Strains and plasmids

From: Renewable synthesis of n-butyraldehyde from glucose by engineered Escherichia coli

Strain Genotype References
BW25113 rrnB T14 ΔlacZ WJ16 hsdR514 ΔaraBAD AH33 ΔrhaBAD LD78  
XL1-blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F’ proAB lacI q ZΔM15 Tn10 (Tetr)] Agilent Technologies
JCL16 BW25113/F’ [traD36 proAB + lacIqZΔM15 (Tetr)] [30]
JCL299 JCL16 ΔadhE ΔldhA ΔfrdBC Δpta [9]
ELeco1 JCL299 ΔyqhD This study
KS1 JCL299 ΔyqhD ΔyjgB This study
KS2 JCL299 ΔyqhD ΔyjgB ΔfucO This study
KS3 JCL299 ΔyqhD ΔyjgB ΔfucO ΔeutG This study
KS4 JCL299 ΔyqhD ΔyjgB ΔfucO ΔeutG ΔybbO This study
KS5 JCL299 ΔyqhD ΔyjgB ΔfucO ΔeutG ΔybbO ΔadhP This study
KS6 JCL299 ΔyqhD ΔyjgB ΔfucO ΔeutG ΔybbO ΔadhP ΔgldA This study
KS7 JCL299 ΔyqhD ΔyjgB ΔfucO ΔeutG ΔybbO ΔadhP ΔgldA ΔyahK This study
KS8 JCL299 ΔyqhD ΔyjgB ΔfucO ΔeutG ΔybbO ΔadhP ΔgldA ΔyahK ΔyghA This study
Plasmid Genotype References
pRW13 Pack::atoB, adhE2, crt, hbd; ColE1 ori; Ampr [19]
pRW18 PadhE::fdh; pSC101 ori; Cmr [19]
pRW22 PadhE::ter; Cola ori; Kanr [19]
pKU48 Pack::atoB, aldh (Clostridium beijerinckii), crt, hbd; ColE1 ori; Ampr This study
pKU49 Pack::atoB, aldh (mutant gene; Clostridium beijerinckii), crt, hbd; ColE1 ori; Ampr This study
pKU50 Pack::atoB, aldh (Clostridium saccharobutylicum), crt, hbd; ColE1 ori; Ampr This study
pKU51 Pack::atoB, aldh (Clostridium saccharoperbutylacetonicum N1-4), crt, hbd; ColE1 ori; Ampr This study
  1. Gene sources are as follows: atoB, acetyl-CoA acetyltransferase from E. coli; adhE2, aldehyde–alcohol dehydrogenase from Clostridium acetobutylicum; crt, crotonase from Clostridium acetobutylicum; hbd, 3-hydroxybutyryl-CoA dehydrogenase from Clostridium acetobutylicum; fdh, formate dehydrogenase from Candida boidinii, ter, trans-enoyl-CoA reductase from Treponema denticola I; aldh, aldehyde dehydrogenase from sources indicated in the table