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Table 2 Calibration statistics for equations generated for prediction of biomass saccharification in transgenetic rice straws

From: A precise and consistent assay for major wall polymer features that distinctively determine biomass saccharification in transgenic rice by near-infrared spectroscopy

 

Calibration

Cross validation

N

Terms

DT

SCM

Spectrum range (nm)

Mean

SD

SEC

R 2

SECV

R 2 cv

RPD

Pretreatment (% total)

 Pentose

140

8

2,5,5,2

DET

1108–2492

9.64

1.26

0.37

0.91

0.42

0.89

2.97

 Hexoses

142

10

1,4,4,1

SNV

780–2492

19.34

7.95

1.16

0.98

1.35

0.97

5.89

 Total

141

10

2,4,4,2

SNVD

780–2492

29.26

6.81

1.00

0.98

1.38

0.96

4.95

Enzymatic hydrolysis (% total)

 Pentose

138

9

2,5,5,2

None

780–2492

23.90

4.01

0.69

0.97

0.83

0.96

4.80

 Hexoses

143

10

2,10,10,2

None

780–2492

46.62

3.40

1.13

0.89

1.33

0.85

2.56

 Total

141

10

2,4,4,2

SNVD

780–2492

70.74

6.81

1.00

0.98

1.38

0.96

4.95

Total sugar released (% dry matter)

 Pentose

141

8

2,10,10,2

DET

1108–2492

15.21

1.59

0.48

0.91

0.54

0.88

2.93

 Hexoses

139

9

2,10,10,2

DET

780–2492

30.38

4.88

1.08

0.95

1.24

0.94

3.95

 Total

138

9

1,4,4,1

WMSCn

1108–2492

45.31

3.97

1.21

0.91

1.38

0.88

2.88

Fermentable hexoses (% total hexoses)

144

9

2,8,8,2

WMSC

408–2492

71.17

10.11

1.78

0.97

2.20

0.95

4.60

  1. N sample number, Terms number of principal component used for calibration, DT derivative treatment, SCM scatter correction methods, SD standard deviation of reference value, SEC standard error of calibration, R 2 determination coefficient, SECV standard error of cross validation, R 2 cv determination coefficient of cross validation, RPD ratio performance deviation (SD/SECV), DET detrend, SNV standard normal variate, SNVD a combination of standard normal variate and detrend, WMSC weighted multiple scatter correction