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Table 3 Calibration statistics for equations generated for prediction of wall polymers and biomass saccharification in transgenetic rice straws

From: A precise and consistent assay for major wall polymer features that distinctively determine biomass saccharification in transgenic rice by near-infrared spectroscopy

  Calibration Cross validation
N Terms DT SCM Spectrum range (nm) Mean SD SEC R 2 SECV R 2 cv RPD
Wall polymers
 Cellulose 234 7 2,5,5,2 WMSC 1108–2492 26.09 4.95 1.56 0.9 1.69 0.88 2.94
 Hemicellulose (% total)
  Ara (%) 236 5 2,4,4,1 None 780–2492 13.25 1.3 0.7 0.71 0.73 0.69 1.79
  Xyl (%) 233 9 2,4,4,2 None 1108–2492 75.16 2.71 1.04 0.85 1.15 0.82 2.35
  X/A 230 8 0,0,1,1 SNV 408–2492 5.73 0.72 0.36 0.75 0.37 0.73 1.94
 Lignin (% dry matter)
  ASL 234 9 2,5,5,2 WMSC 780–2492 3.12 0.38 0.14 0.87 0.16 0.83 2.45
  AIL 231 7 2,4,4,1 SNVD 1108–2492 8.34 1.34 0.69 0.74 0.74 0.69 1.81
  Total 231 7 0,0,1,1 SNVD 408–2492 11.46 1.47 0.71 0.77 0.74 0.74 1.98
Biomass saccharification
 Pretreatment (% total)
  Pentose 234 7 2,4,4,2 MSC 1108–2492 9.78 1.24 0.43 0.88 0.47 0.86 2.63
  Hexoses 228 8 2,8,8,1 SNV 1108–2492 18.6 7.58 1.08 0.98 1.14 0.98 6.63
  Total 230 12 2,8,8,1 WMSC 780–2492 28.57 6.54 1.02 0.98 1.19 0.97 5.48
 Enzymatic hydrolysis (% total)
  Pentose 230 11 2,10,10,2 None 1108–2492 24.37 3.84 0.74 0.96 0.81 0.96 4.74
  Hexoses 233 10 1,4,4,1 DET 408–2492 46.94 3.19 1.15 0.87 1.26 0.85 2.54
  Total 230 12 2,8,8,1 WMSC 780–2492 71.43 6.54 1.02 0.98 1.19 0.97 5.48
 Total released sugar (% dry matter)
  Pentose 234 12 1,4,4,1 None 1108–2492 15.35 1.54 0.46 0.91 0.51 0.89 3.05
  Hexoses 232 11 2,8,8,1 WMSC 1108–2492 29.89 4.62 1.2 0.93 1.4 0.91 3.3
  Total 233 11 2,5,5,2 None 1108–2492 45.21 3.94 1.35 0.88 1.65 0.83 2.39
Fermentable hexoses (% total hexoses) 235 9 1,4,4,1 SNVD 408–2492 72.03 9.65 1.76 0.97 1.86 0.96 5.19
  1. N sample number, Terms number of principal component used for calibration, DT derivative treatment, SCM scatter correction methods, SD standard deviation of reference value, SEC standard error of calibration, R 2 determination coefficient, SECV standard error of cross validation, R 2 cv determination coefficient of cross validation, RPD ratio performance deviation (SD/SECV), WMSC weighted multiple scatter correction, SNV standard normal variate, SNVD a combination of standard normal variate and detrend, MSC standard multiple scatter, DET detrend, Ara arabinose, Xyl xylose, X/A xylose/arabinose, ASL acid soluble lignin, AIL acid insoluble lignin