A precise and consistent assay for major wall polymer features that distinctively determine biomass saccharification in transgenic rice by near-infrared spectroscopy

Table 3 Calibration statistics for equations generated for prediction of wall polymers and biomass saccharification in transgenetic rice straws

	Calibration									Cross validation
	N	Terms	DT	SCM	Spectrum range (nm)	Mean	SD	SEC	R ²	SECV	R ² _cv	RPD
Wall polymers
Cellulose	234	7	2,5,5,2	WMSC	1108–2492	26.09	4.95	1.56	0.9	1.69	0.88	2.94
Hemicellulose (% total)
Ara (%)	236	5	2,4,4,1	None	780–2492	13.25	1.3	0.7	0.71	0.73	0.69	1.79
Xyl (%)	233	9	2,4,4,2	None	1108–2492	75.16	2.71	1.04	0.85	1.15	0.82	2.35
X/A	230	8	0,0,1,1	SNV	408–2492	5.73	0.72	0.36	0.75	0.37	0.73	1.94
Lignin (% dry matter)
ASL	234	9	2,5,5,2	WMSC	780–2492	3.12	0.38	0.14	0.87	0.16	0.83	2.45
AIL	231	7	2,4,4,1	SNVD	1108–2492	8.34	1.34	0.69	0.74	0.74	0.69	1.81
Total	231	7	0,0,1,1	SNVD	408–2492	11.46	1.47	0.71	0.77	0.74	0.74	1.98
Biomass saccharification
Pretreatment (% total)
Pentose	234	7	2,4,4,2	MSC	1108–2492	9.78	1.24	0.43	0.88	0.47	0.86	2.63
Hexoses	228	8	2,8,8,1	SNV	1108–2492	18.6	7.58	1.08	0.98	1.14	0.98	6.63
Total	230	12	2,8,8,1	WMSC	780–2492	28.57	6.54	1.02	0.98	1.19	0.97	5.48
Enzymatic hydrolysis (% total)
Pentose	230	11	2,10,10,2	None	1108–2492	24.37	3.84	0.74	0.96	0.81	0.96	4.74
Hexoses	233	10	1,4,4,1	DET	408–2492	46.94	3.19	1.15	0.87	1.26	0.85	2.54
Total	230	12	2,8,8,1	WMSC	780–2492	71.43	6.54	1.02	0.98	1.19	0.97	5.48
Total released sugar (% dry matter)
Pentose	234	12	1,4,4,1	None	1108–2492	15.35	1.54	0.46	0.91	0.51	0.89	3.05
Hexoses	232	11	2,8,8,1	WMSC	1108–2492	29.89	4.62	1.2	0.93	1.4	0.91	3.3
Total	233	11	2,5,5,2	None	1108–2492	45.21	3.94	1.35	0.88	1.65	0.83	2.39
Fermentable hexoses (% total hexoses)	235	9	1,4,4,1	SNVD	408–2492	72.03	9.65	1.76	0.97	1.86	0.96	5.19

N sample number, Terms number of principal component used for calibration, DT derivative treatment, SCM scatter correction methods, SD standard deviation of reference value, SEC standard error of calibration, R ² determination coefficient, SECV standard error of cross validation, R ² _cv determination coefficient of cross validation, RPD ratio performance deviation (SD/SECV), WMSC weighted multiple scatter correction, SNV standard normal variate, SNVD a combination of standard normal variate and detrend, MSC standard multiple scatter, DET detrend, Ara arabinose, Xyl xylose, X/A xylose/arabinose, ASL acid soluble lignin, AIL acid insoluble lignin

ISSN: 2731-3654