Expressing accessory proteins in cellulolytic Yarrowia lipolytica to improve the conversion yield of recalcitrant cellulose

Table 2 Comparison of cellulose utilization and biomass yield of cellulolytic Y. lipolytica grown on different cellulosic substrates for 120 h in aerobic cultivation

Strains	CIMV-cellulose consumed %	Biomass yield %	Avicel consumed %	Biomass yield %	Cellulose consumed % (wheat straw)	Biomass yield %
YLC6	58.6 ± 0.5	0.41 ± 0.03	30.2 ± 0.3	0.32 ± 0.03	9.7 ± 0.6	0.12 ± 0.01
YLC7	61.5 ± 0.6	0.40 ± 0.05	32.0 ± 0.5	0.31 ± 0.02	10.5 ± 0.7	0.11 ± 0.01
YLC8	64.0 ± 0.4	0.40 ± 0.02	31.8 ± 1.0	0.31 ± 0.02	12.3 ± 0.5	0.12 ± 0.01
YLC9	65.4 ± 0.7	0.39 ± 0.03	36.3 ± 0.9	0.29 ± 0.03	15.8 ± 0.9	0.15 ± 0.01
YLC10	59.0 ± 1.5	0.40 ± 0.04	31.0 ± 1.2	0.31 ± 0.04	10.3 ± 0.9	0.11 ± 0.02
YLC11	67.8 ± 1.0	0.39 ± 0.02	36.5 ± 0.7	0.29 ± 0.02	18.4 ± 0.8	0.15 ± 0.02
YLC11 + S^a	68.3 ± 1.3	0.38 ± 0.01	42.0 ± 1.1	0.30 ± 0.04	21.6 ± 0.5	0.17 ± 0.01

The results were calculated from at least three biological replicates, and are given as the mean value ± standard deviation. The initial cellulose content was 25 g/L for all the substrates
^aThe cellulose was treated by SWO1 at the dosage of 15 mg/g cellulose for 24 h before enzymatic hydrolysis

ISSN: 2731-3654